Effect on plasma lipids of interesterifying a mix of edible oils

Paul J. Nestel, Manny Noakes, G. Bryan Belling, Rosemary McArthur, Peter M. Clifton

Research output: Contribution to journalArticle

54 Citations (Scopus)


The aim was to establish whether interesterification of oils, an alternative to the use of trans fatty acids in margarine manufacture, adversely affects plasma lipids. Twenty-seven hypercholesterolemic men participated in a double-blind, crossover trial of three margarines: 1) high- linoleic acid, moderate trans fatty acids; 2) high-palm oil blend (predominantly lauric, myristic, palmitic, oleic, and linoleic acids); and 3) an interesterified form of the high-palm oil margarine. Both high-palm oil margarines led to similar low-density-lipoprotein (LDL) cholesterol concentrations (4.43 ± 0.94 and 4.54 ± 0.88 mmol/L, respectively), which were significantly higher than the LDL concentrations after the high-linoleic acid margarine (4.02 ± 0.85 mmol/L, P < 0.001). Interesterification transferred substantial proportions of palmitic acid into the sn-2 position of glycerides and unsaturated fatty acids into the sn-1,3 positions, and this was reflected in plasma chylomicrons. This study shows that interesterification of oils used to harden margarines does not raise plasma cholesterol more than does the margarine's constituent fatty acids.

Original languageEnglish
Pages (from-to)950-955
Number of pages6
JournalAmerican Journal of Clinical Nutrition
Issue number5
Publication statusPublished - 1 Jan 1995
Externally publishedYes


  • Low-density-lipoprotein cholesterol
  • chylomicrons
  • fatty acids
  • interesterification

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Nestel, P. J., Noakes, M., Belling, G. B., McArthur, R., & Clifton, P. M. (1995). Effect on plasma lipids of interesterifying a mix of edible oils. American Journal of Clinical Nutrition, 62(5), 950-955. https://doi.org/10.1093/ajcn/62.5.950