Effect of voltage-gated and capacitative calcium entry blockade on agonist-induced constriction of equine laminar blood vessels

John F. Peroni, James N. Moore, Erik Noschka, Tristan H. Lewis, Stephen J. Lewis, Tom P. Roberston

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Abstract

Objective - To characterize the relative contributions of voltage-gated and capacitative Ca2+ entry to agonist-induced contractions of equine laminar arteries and veins. Animals - 16 adult mixed-breed horses. Procedures - Laminar arteries and veins were isolated and mounted on small vessel myographs for the measurement of isometric tension. Concentration-response curves were obtained for the vasoconstrictor agonists phenylephrine, 5-hydroxytryptamine (5-HT), prostaglandin F (PGF), and endothelin-1 (ET-1) either in the absence of extracellular Ca2+ or in the presence of the voltage-gated Ca2+ channel inhibitor diltiazem or the putative inhibitor of capacitative Ca2+ entry, trifluoromethylphenylimidazole. Results - In the absence of extracellular Ca2+, maximal responses of veins to 5-HT, phenylephrine, ET-1 and PGF were reduced by 80%, 50%, 50%, and 45%, respectively; responses of arteries to 5-HT, phenylephrine, and ET-1 were reduced by 95%, 90%, and 20%, respectively. Although diltiazem did not affect the maximal responses of veins to any agonist, responses of arteries to 5-HT phenylephrine, and ET-1 were reduced by 40%, 50%, and 27%, respectively. Trifluoromethylphenylimidazole did not affect maximal responses of veins, but did reduce their contractile responses to low concentrations of ET-1 and PGF. Conclusions and Clinical Relevance - Results suggested that the contribution of extracellular Ca2+ to laminar vessel contractile responses differs between arteries and veins and also between contractile agonists, voltage-gated Ca2+ entry is more predominant in laminar arteries than in veins, and capacitative Ca2+ entry has a minor role in agonist-induced contractile responses of laminar veins.

Original languageEnglish
Pages (from-to)722-729
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume68
Issue number7
DOIs
Publication statusPublished - 1 Jul 2007
Externally publishedYes

ASJC Scopus subject areas

  • veterinary(all)

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