Effect of n-3 and n-6 dietary fats on the lipoxygenase products from stimulated rat neutrophils

R. A. Gibson, M. A. Neumann, M. J. James, J. S. Hawkes, C. Hall, L. G. Cleland

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Fish oil was fed to rats in combination with an equal amount of olive, sunflower or linseed (flax) oil in semisynthetic diets for 3 weeks. Following stimulation of isolated neutrophils with calcium ionophore the levels of leukotrienes (LT) were determined by HPLC. Graphical presentation of the resultant data show a direct linear relationship between LTB production and substrate concentration with no preferential conversion of n-3 or n-6 substrates. In addition the results highlighted the greater conversion of eicosapentaenoic acid (EPA) and arachidonic acid (AA) to 5-hydroxy metabolites in stimulated neutrophils. There is no suggestion in our results of inhibition of any of the enzymatic conversion steps between EPA or AA and LTB production by any of the dietary fatty acids except by altering the EPA/AA ratio in neutrophil membranes.

LanguageEnglish
Pages87-91
Number of pages5
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume46
Issue number2
DOIs
Publication statusPublished - 1 Jan 1992

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this

Gibson, R. A. ; Neumann, M. A. ; James, M. J. ; Hawkes, J. S. ; Hall, C. ; Cleland, L. G. / Effect of n-3 and n-6 dietary fats on the lipoxygenase products from stimulated rat neutrophils. In: Prostaglandins, Leukotrienes and Essential Fatty Acids. 1992 ; Vol. 46, No. 2. pp. 87-91.
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Effect of n-3 and n-6 dietary fats on the lipoxygenase products from stimulated rat neutrophils. / Gibson, R. A.; Neumann, M. A.; James, M. J.; Hawkes, J. S.; Hall, C.; Cleland, L. G.

In: Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 46, No. 2, 01.01.1992, p. 87-91.

Research output: Contribution to journalArticle

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