Effect of maternal docosahexaenoic acid (DHA) supplementation on breast milk composition

M. Makrides, M. A. Neumann, R. A. Gibson

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Objective: To assess the effect of varying maternal intake of docosahexaenoic acid (DHA, 22:6n-3), in the absence of other dietary polyunsaturates, on breast milk fatty acids. Design and intervention: Lactating mothers were randomised on day 5 post-partum to groups consuming equal numbers of capsules but containing either placebo or an oil containing DHA (43%) as its only polyunsaturate to receive 0, 0.2, 0.4, 0.9, 1.3 g DHA/day. Breast milk fatty acids as well as maternal plasma and erythrocyte phospholipids were assessed at 12 weeks post partum by capillary gas chromatography. Results: Breast milk DHA levels ranged from 0.2 to 1.7% of total fatty acids and increased in a dose dependent manner (r2 = 0.89, P < 0.01). Maternal plasma (r2 = 0.71, P < 0.01) and erythrocyte (r2 = 0.77, P < 0.01) phospholipid DHA levels increased and were also strongly associated with dietary dose of DHA. Increasing maternal dietary doses of DHA did not affect breast milk arachidonic acid (AA, 20:4n-6) levels or antioxidant status as measured by plasma vitamin A or E levels. Conclusions: Our results have demonstrated that DHA in the diet has a strong, specific and dose-dependent effect on breast milk DHA.

Original languageEnglish
Pages (from-to)352-357
Number of pages6
JournalEuropean Journal of Clinical Nutrition
Issue number6
Publication statusPublished - 1 Jul 1996
Externally publishedYes


  • Breast milk
  • Docosahexaenoic acid (DHA)
  • Maternal diet

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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