Abstract
Background: Maternal avoidance of egg intake has been recommended to treat egg allergy in breastfed infants. Objective: To determine if the concentration of ovalbumin (OVA) in human milk is directly related to the quantity and form of egg consumed by breastfeeding mothers. Methods: Randomized, blinded, cross-over, intervention trial. Breastfeeding women (n = 41) attended four clinic days between 11 and 14 weeks of lactation and on each day were randomly allocated to receive a test breakfast, identical except for the egg content (no egg, one raw egg, half a cooked egg or one cooked egg). Breast milk samples were collected at two hourly intervals for 8 h and their OVA concentration measured by ELISA. Results: There was a direct, dose-response between the amount of cooked egg ingested and the peak OVA concentration (no egg 0.05ng/mL [95% confidence interval (CI), 0.01-0.11], half a cooked egg 2.24 ng/mL [95% CI, 0.57-3.91], one cooked egg 3.16 ng/mL [95% CI, 1.41-4.91], n = 41, P < 0.05) as well as the total OVA excretion (no egg 0.18 ng/mL/h [95% CI, 0.04-0.39], half a cooked egg 4.93 ng/mL/h [95% CI, 1.40-8.46], one cooked egg 9.14 ng/mL/h [95% CI, 4.25-14.03], n = 41, P < 0.05). The peak concentration and total OVA excretion in response to one raw egg did not differ from ingesting half a cooked egg. There was no detectable OVA in the breast milk of 24% (10/41) women up to 8 h after any egg challenge. Conclusion: OVA was detected in the breast milk of lactating women up to 8 h after a controlled intake of egg. A dose-response correlation was indicated. As excretion of OVA in human milk appears to be a normal phenomenon, further studies need to determine the threshold of OVA excretion that leads to symptoms in egg-allergic breastfed infants.
| Language | English |
|---|---|
| Pages | 173-178 |
| Number of pages | 6 |
| Journal | Clinical and Experimental Allergy |
| Volume | 35 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Feb 2005 |
| Externally published | Yes |
Keywords
- Egg
- Human milk
- Maternal antigen avoidance diet
- Ovalbumin
- Randomised controlled trial
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
}
Effect of cooked and raw egg consumption on ovalbumin content of human milk : A randomized, double-blind, cross-over trial. / Palmer, D. J.; Gold, M. S.; Makrides, Maria.
In: Clinical and Experimental Allergy, Vol. 35, No. 2, 01.02.2005, p. 173-178.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effect of cooked and raw egg consumption on ovalbumin content of human milk
T2 - Clinical and Experimental Allergy
AU - Palmer, D. J.
AU - Gold, M. S.
AU - Makrides, Maria
PY - 2005/2/1
Y1 - 2005/2/1
N2 - Background: Maternal avoidance of egg intake has been recommended to treat egg allergy in breastfed infants. Objective: To determine if the concentration of ovalbumin (OVA) in human milk is directly related to the quantity and form of egg consumed by breastfeeding mothers. Methods: Randomized, blinded, cross-over, intervention trial. Breastfeeding women (n = 41) attended four clinic days between 11 and 14 weeks of lactation and on each day were randomly allocated to receive a test breakfast, identical except for the egg content (no egg, one raw egg, half a cooked egg or one cooked egg). Breast milk samples were collected at two hourly intervals for 8 h and their OVA concentration measured by ELISA. Results: There was a direct, dose-response between the amount of cooked egg ingested and the peak OVA concentration (no egg 0.05ng/mL [95% confidence interval (CI), 0.01-0.11], half a cooked egg 2.24 ng/mL [95% CI, 0.57-3.91], one cooked egg 3.16 ng/mL [95% CI, 1.41-4.91], n = 41, P < 0.05) as well as the total OVA excretion (no egg 0.18 ng/mL/h [95% CI, 0.04-0.39], half a cooked egg 4.93 ng/mL/h [95% CI, 1.40-8.46], one cooked egg 9.14 ng/mL/h [95% CI, 4.25-14.03], n = 41, P < 0.05). The peak concentration and total OVA excretion in response to one raw egg did not differ from ingesting half a cooked egg. There was no detectable OVA in the breast milk of 24% (10/41) women up to 8 h after any egg challenge. Conclusion: OVA was detected in the breast milk of lactating women up to 8 h after a controlled intake of egg. A dose-response correlation was indicated. As excretion of OVA in human milk appears to be a normal phenomenon, further studies need to determine the threshold of OVA excretion that leads to symptoms in egg-allergic breastfed infants.
AB - Background: Maternal avoidance of egg intake has been recommended to treat egg allergy in breastfed infants. Objective: To determine if the concentration of ovalbumin (OVA) in human milk is directly related to the quantity and form of egg consumed by breastfeeding mothers. Methods: Randomized, blinded, cross-over, intervention trial. Breastfeeding women (n = 41) attended four clinic days between 11 and 14 weeks of lactation and on each day were randomly allocated to receive a test breakfast, identical except for the egg content (no egg, one raw egg, half a cooked egg or one cooked egg). Breast milk samples were collected at two hourly intervals for 8 h and their OVA concentration measured by ELISA. Results: There was a direct, dose-response between the amount of cooked egg ingested and the peak OVA concentration (no egg 0.05ng/mL [95% confidence interval (CI), 0.01-0.11], half a cooked egg 2.24 ng/mL [95% CI, 0.57-3.91], one cooked egg 3.16 ng/mL [95% CI, 1.41-4.91], n = 41, P < 0.05) as well as the total OVA excretion (no egg 0.18 ng/mL/h [95% CI, 0.04-0.39], half a cooked egg 4.93 ng/mL/h [95% CI, 1.40-8.46], one cooked egg 9.14 ng/mL/h [95% CI, 4.25-14.03], n = 41, P < 0.05). The peak concentration and total OVA excretion in response to one raw egg did not differ from ingesting half a cooked egg. There was no detectable OVA in the breast milk of 24% (10/41) women up to 8 h after any egg challenge. Conclusion: OVA was detected in the breast milk of lactating women up to 8 h after a controlled intake of egg. A dose-response correlation was indicated. As excretion of OVA in human milk appears to be a normal phenomenon, further studies need to determine the threshold of OVA excretion that leads to symptoms in egg-allergic breastfed infants.
KW - Egg
KW - Human milk
KW - Maternal antigen avoidance diet
KW - Ovalbumin
KW - Randomised controlled trial
UR - http://www.scopus.com/inward/record.url?scp=14844340205&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2222.2005.02170.x
DO - 10.1111/j.1365-2222.2005.02170.x
M3 - Article
VL - 35
SP - 173
EP - 178
JO - Clinical and Experimental Allergy
JF - Clinical and Experimental Allergy
SN - 0954-7894
IS - 2
ER -