Duodenal fatty acid sensor and transporter expression following acute fat exposure in healthy lean humans

Nada Cvijanovic, Nicole Isaacs, Christopher K. Rayner, Christine Feinle-Bisset, Richard Young, Tanya J. Little

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background & aims Free fatty acids (FFAs) and their derivatives are detected by G-protein coupled receptors (GPRs) on enteroendocrine cells, with specific transporters on enterocytes. It is unknown whether acute fat exposure affects FFA sensors/transporters, and whether this relates to hormone secretion and habitual fat intake. Methods We studied 20 healthy participants (10M, 10F; BMI: 22 ± 1 kg/m2; age: 28 ± 2 years), after an overnight fast, on 2 separate days. On the first day, duodenal biopsies were collected endoscopically before, and after, a 30-min intraduodenal (ID) infusion of 10% Intralipid®, and relative transcript expression of FFA receptor 1 (FFAR1), FFA receptor 4 (FFAR4), GPR119 and the FFA transporter, cluster of differentiation-36 (CD36) was quantified from biopsies. On the second day, ID Intralipid® was infused for 120-min, and plasma concentrations of cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) evaluated. Habitual dietary intake was assessed using food frequency questionnaires (FFQs). Results ID Intralipid® increased expression of GPR119, but not FFAR1, FFAR4 and CD36, and stimulated CCK and GLP-1 secretion. Habitual polyunsaturated fatty acid (PUFA) consumption was negatively associated with basal GPR119 expression. Conclusions GPR119 is an early transcriptional responder to duodenal lipid in lean humans, although this response appeared reduced in individuals with high PUFA intake. These observations may have implications for downstream regulation of gut hormone secretion and appetite. This study was registered as a clinical trial with the Australia and New Zealand Clinical Trial Registry (Trial number: ACTRN12612000376842).

LanguageEnglish
Pages564-569
Number of pages6
JournalClinical Nutrition
Volume36
Issue number2
DOIs
Publication statusPublished - 1 Apr 2017

Keywords

  • CD36
  • Cholecystokinin
  • Free fatty acid receptor 1
  • Free fatty acid receptor 4
  • G-protein coupled receptor 119
  • Glucagon-like peptide-1

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Critical Care and Intensive Care Medicine

Cite this

Cvijanovic, Nada ; Isaacs, Nicole ; Rayner, Christopher K. ; Feinle-Bisset, Christine ; Young, Richard ; Little, Tanya J. / Duodenal fatty acid sensor and transporter expression following acute fat exposure in healthy lean humans. In: Clinical Nutrition. 2017 ; Vol. 36, No. 2. pp. 564-569.
@article{99f34447ba5c4f7e82e08665c5342373,
title = "Duodenal fatty acid sensor and transporter expression following acute fat exposure in healthy lean humans",
abstract = "Background & aims Free fatty acids (FFAs) and their derivatives are detected by G-protein coupled receptors (GPRs) on enteroendocrine cells, with specific transporters on enterocytes. It is unknown whether acute fat exposure affects FFA sensors/transporters, and whether this relates to hormone secretion and habitual fat intake. Methods We studied 20 healthy participants (10M, 10F; BMI: 22 ± 1 kg/m2; age: 28 ± 2 years), after an overnight fast, on 2 separate days. On the first day, duodenal biopsies were collected endoscopically before, and after, a 30-min intraduodenal (ID) infusion of 10{\%} Intralipid{\circledR}, and relative transcript expression of FFA receptor 1 (FFAR1), FFA receptor 4 (FFAR4), GPR119 and the FFA transporter, cluster of differentiation-36 (CD36) was quantified from biopsies. On the second day, ID Intralipid{\circledR} was infused for 120-min, and plasma concentrations of cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) evaluated. Habitual dietary intake was assessed using food frequency questionnaires (FFQs). Results ID Intralipid{\circledR} increased expression of GPR119, but not FFAR1, FFAR4 and CD36, and stimulated CCK and GLP-1 secretion. Habitual polyunsaturated fatty acid (PUFA) consumption was negatively associated with basal GPR119 expression. Conclusions GPR119 is an early transcriptional responder to duodenal lipid in lean humans, although this response appeared reduced in individuals with high PUFA intake. These observations may have implications for downstream regulation of gut hormone secretion and appetite. This study was registered as a clinical trial with the Australia and New Zealand Clinical Trial Registry (Trial number: ACTRN12612000376842).",
keywords = "CD36, Cholecystokinin, Free fatty acid receptor 1, Free fatty acid receptor 4, G-protein coupled receptor 119, Glucagon-like peptide-1",
author = "Nada Cvijanovic and Nicole Isaacs and Rayner, {Christopher K.} and Christine Feinle-Bisset and Richard Young and Little, {Tanya J.}",
year = "2017",
month = "4",
day = "1",
doi = "10.1016/j.clnu.2016.02.005",
language = "English",
volume = "36",
pages = "564--569",
journal = "Clinical Nutrition",
issn = "0261-5614",
publisher = "Churchill Livingstone",
number = "2",

}

Duodenal fatty acid sensor and transporter expression following acute fat exposure in healthy lean humans. / Cvijanovic, Nada; Isaacs, Nicole; Rayner, Christopher K.; Feinle-Bisset, Christine; Young, Richard; Little, Tanya J.

In: Clinical Nutrition, Vol. 36, No. 2, 01.04.2017, p. 564-569.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Duodenal fatty acid sensor and transporter expression following acute fat exposure in healthy lean humans

AU - Cvijanovic, Nada

AU - Isaacs, Nicole

AU - Rayner, Christopher K.

AU - Feinle-Bisset, Christine

AU - Young, Richard

AU - Little, Tanya J.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Background & aims Free fatty acids (FFAs) and their derivatives are detected by G-protein coupled receptors (GPRs) on enteroendocrine cells, with specific transporters on enterocytes. It is unknown whether acute fat exposure affects FFA sensors/transporters, and whether this relates to hormone secretion and habitual fat intake. Methods We studied 20 healthy participants (10M, 10F; BMI: 22 ± 1 kg/m2; age: 28 ± 2 years), after an overnight fast, on 2 separate days. On the first day, duodenal biopsies were collected endoscopically before, and after, a 30-min intraduodenal (ID) infusion of 10% Intralipid®, and relative transcript expression of FFA receptor 1 (FFAR1), FFA receptor 4 (FFAR4), GPR119 and the FFA transporter, cluster of differentiation-36 (CD36) was quantified from biopsies. On the second day, ID Intralipid® was infused for 120-min, and plasma concentrations of cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) evaluated. Habitual dietary intake was assessed using food frequency questionnaires (FFQs). Results ID Intralipid® increased expression of GPR119, but not FFAR1, FFAR4 and CD36, and stimulated CCK and GLP-1 secretion. Habitual polyunsaturated fatty acid (PUFA) consumption was negatively associated with basal GPR119 expression. Conclusions GPR119 is an early transcriptional responder to duodenal lipid in lean humans, although this response appeared reduced in individuals with high PUFA intake. These observations may have implications for downstream regulation of gut hormone secretion and appetite. This study was registered as a clinical trial with the Australia and New Zealand Clinical Trial Registry (Trial number: ACTRN12612000376842).

AB - Background & aims Free fatty acids (FFAs) and their derivatives are detected by G-protein coupled receptors (GPRs) on enteroendocrine cells, with specific transporters on enterocytes. It is unknown whether acute fat exposure affects FFA sensors/transporters, and whether this relates to hormone secretion and habitual fat intake. Methods We studied 20 healthy participants (10M, 10F; BMI: 22 ± 1 kg/m2; age: 28 ± 2 years), after an overnight fast, on 2 separate days. On the first day, duodenal biopsies were collected endoscopically before, and after, a 30-min intraduodenal (ID) infusion of 10% Intralipid®, and relative transcript expression of FFA receptor 1 (FFAR1), FFA receptor 4 (FFAR4), GPR119 and the FFA transporter, cluster of differentiation-36 (CD36) was quantified from biopsies. On the second day, ID Intralipid® was infused for 120-min, and plasma concentrations of cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) evaluated. Habitual dietary intake was assessed using food frequency questionnaires (FFQs). Results ID Intralipid® increased expression of GPR119, but not FFAR1, FFAR4 and CD36, and stimulated CCK and GLP-1 secretion. Habitual polyunsaturated fatty acid (PUFA) consumption was negatively associated with basal GPR119 expression. Conclusions GPR119 is an early transcriptional responder to duodenal lipid in lean humans, although this response appeared reduced in individuals with high PUFA intake. These observations may have implications for downstream regulation of gut hormone secretion and appetite. This study was registered as a clinical trial with the Australia and New Zealand Clinical Trial Registry (Trial number: ACTRN12612000376842).

KW - CD36

KW - Cholecystokinin

KW - Free fatty acid receptor 1

KW - Free fatty acid receptor 4

KW - G-protein coupled receptor 119

KW - Glucagon-like peptide-1

UR - http://www.scopus.com/inward/record.url?scp=84959187722&partnerID=8YFLogxK

U2 - 10.1016/j.clnu.2016.02.005

DO - 10.1016/j.clnu.2016.02.005

M3 - Article

VL - 36

SP - 564

EP - 569

JO - Clinical Nutrition

T2 - Clinical Nutrition

JF - Clinical Nutrition

SN - 0261-5614

IS - 2

ER -