Distribution and quantitation of hla-abc and dr (ia) antigens on human kidney and other tissues

Keryn Williams, Derek N.J. Hart, John W. Fabre, Peter J. Morris

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

A quantitative estimation of the amounts of human la (HLADR) and HLA-ABC antigen on a variety of human tissues was performed. Monoclonal antibodies to species-common determinants of HLA-DR and HLA-ABC antigens were absorbed quantitatively with tissue homogenates and cell suspensions, and reassayed for residual activity in a radioimmunobinding assay. Kidney was found to carry 90% as much HLA-DR and 14% as much HLA-ABC antigen as spleen, while liver contained 19 and 9% as much, respectively. Small amounts of both antigens were found on heart; brain carried very little HLA-ABC and virtually no DR. Neither HLA-ABC nor DR was found on erythrocytes or reticulocytes. Of interest was our finding that a small subpopulation of thymocytes (10%) was HLA-DR positive. Platelets contained approximately 5% of the amount of HLA-ABC as spleen and undetectable quantities of HLA-DR, as expected. Chronic lymphatic leukemia (CLL) cells were found to carry 10% as much HLA-ABC and 33% as much DR antigen as spleen, while the values for bone marrow were 15 and 2%, respectively.

LanguageEnglish
Pages274-279
Number of pages6
JournalTransplantation
Volume29
Issue number4
DOIs
Publication statusPublished - 1 Jan 1980

ASJC Scopus subject areas

  • Transplantation

Cite this

Williams, Keryn ; Hart, Derek N.J. ; Fabre, John W. ; Morris, Peter J. / Distribution and quantitation of hla-abc and dr (ia) antigens on human kidney and other tissues. In: Transplantation. 1980 ; Vol. 29, No. 4. pp. 274-279.
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Distribution and quantitation of hla-abc and dr (ia) antigens on human kidney and other tissues. / Williams, Keryn; Hart, Derek N.J.; Fabre, John W.; Morris, Peter J.

In: Transplantation, Vol. 29, No. 4, 01.01.1980, p. 274-279.

Research output: Contribution to journalArticle

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