Differentiation of iPSC to mesenchymal stem-like cells and their characterization

Kim Hynes, Danijela Menicanin, Stan Gronthos, Mark P. Bartold

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

14 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSC) are a unique population of adult stem cells that have the capacity to differentiate into numerous cell types as well as the ability to modulate the immune system. As such, MSC represent a promising stem cell population for use in the clinical treatment of a range of disorders involving tissue regeneration as well as the immune system. The lack of accessibility to MSC is currently limiting the use of MSC in mainstream clinical treatment strategies. It is therefore imperative for the future success of stem cell-based treatment approaches that are more reliable, and accessible sources of MSC are identified. The present chapter describes a method for generating MSC-like cells from induced pluripotent stem cells (iPSC), with equivalent growth and functional properties to parental MSC populations.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages353-374
Number of pages22
DOIs
Publication statusPublished or Issued - 1 Jan 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1357
ISSN (Print)1064-3745

Keywords

  • Characterization
  • Differentiation
  • Induced pluripotent stem cells
  • Mesenchymal stem cells
  • Serial passaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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