Differential effects of maternal obesity and weight loss in the periconceptional period on the epigenetic regulation of hepatic insulin-signaling pathways in the offspring

Lisa M. Nicholas, Leewen Rattanatray, Severence M. MacLaughlin, Susan E. Ozanne, Dave O. Kleemann, Simon K. Walker, Janna L. Morrison, Song Zhang, Beverley S. Muhlhäusler, Malgorzata S. Martin-Gronert, Isabella C. McMillen

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Our aim was to determine the effect of exposure to maternal obesity or to maternal weight loss around conception on the programming of hepatic insulin signaling in the offspring. We used an embryo transfer model in sheep to investigate the effects of exposure to either maternal obesity or to weight loss in normal and obese mothers preceding and for 1 wk after conception on the expression of hepatic insulin-signaling and gluconeogenic factors and key miRNAs involved in insulin signaling in the offspring. We found that exposure to maternal obesity resulted in increased hepatic miR-29b (P<0.05), miR-103 (P<0.01), and miR-107 (P<0.05) expression, a decrease in IR (P<0.05), phopsho- Akt (P<0.01), and phospho-FoxO1 (P<0.01) abundance, and a paradoxical decrease in 11βHSD1 (P<0.05), PEPCK-C (P<0.01), and PEPCK-M (P<0.05) expression in lambs. These changes were ablated by a period of moderate dietary restriction imposed during the periconceptional period. Maternal dietary restriction alone also resulted in decreased abundance of a separate subset of hepatic insulin-signaling molecules, namely, IRS1 (P<0.05), PDK1 (P<0.01), phospho-PDK1 (P<0.05), and aPKC ζ (P<0.05) and in decreased PEPCK-C (P<0.01) and G6Pase (P<0.01) expression in the lamb. Our findings highlight the sensitivity of the epigenome to maternal nutrition around conception and the need for dietary interventions that maximize metabolic benefits and minimize metabolic costs for the next generation.-Nicholas, L. M., Rattanatray, L., MacLaughlin, S. M., Ozanne, S. E., Kleemann, D. O.,Walker, S. K., Morrison, J. L., Zhang, S., Muhlhausler, B. S., Martin-Gronert, M. S., McMillen, I. C. Differential effects of maternal obesity and weight loss in the periconceptional period on the epigenetic regulation of hepatic insulin-signaling pathways in the offspring.

LanguageEnglish
Pages3786-3796
Number of pages11
JournalFASEB Journal
Volume27
Issue number9
DOIs
Publication statusPublished - Sep 2013
Externally publishedYes

Keywords

  • Diabetes
  • Embryo
  • Fetus
  • MicroRNAs
  • Pregnancy
  • Programming

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Nicholas, L. M., Rattanatray, L., MacLaughlin, S. M., Ozanne, S. E., Kleemann, D. O., Walker, S. K., ... McMillen, I. C. (2013). Differential effects of maternal obesity and weight loss in the periconceptional period on the epigenetic regulation of hepatic insulin-signaling pathways in the offspring. FASEB Journal, 27(9), 3786-3796. https://doi.org/10.1096/fj.13-227918
Nicholas, Lisa M. ; Rattanatray, Leewen ; MacLaughlin, Severence M. ; Ozanne, Susan E. ; Kleemann, Dave O. ; Walker, Simon K. ; Morrison, Janna L. ; Zhang, Song ; Muhlhäusler, Beverley S. ; Martin-Gronert, Malgorzata S. ; McMillen, Isabella C. / Differential effects of maternal obesity and weight loss in the periconceptional period on the epigenetic regulation of hepatic insulin-signaling pathways in the offspring. In: FASEB Journal. 2013 ; Vol. 27, No. 9. pp. 3786-3796.
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abstract = "Our aim was to determine the effect of exposure to maternal obesity or to maternal weight loss around conception on the programming of hepatic insulin signaling in the offspring. We used an embryo transfer model in sheep to investigate the effects of exposure to either maternal obesity or to weight loss in normal and obese mothers preceding and for 1 wk after conception on the expression of hepatic insulin-signaling and gluconeogenic factors and key miRNAs involved in insulin signaling in the offspring. We found that exposure to maternal obesity resulted in increased hepatic miR-29b (P<0.05), miR-103 (P<0.01), and miR-107 (P<0.05) expression, a decrease in IR (P<0.05), phopsho- Akt (P<0.01), and phospho-FoxO1 (P<0.01) abundance, and a paradoxical decrease in 11βHSD1 (P<0.05), PEPCK-C (P<0.01), and PEPCK-M (P<0.05) expression in lambs. These changes were ablated by a period of moderate dietary restriction imposed during the periconceptional period. Maternal dietary restriction alone also resulted in decreased abundance of a separate subset of hepatic insulin-signaling molecules, namely, IRS1 (P<0.05), PDK1 (P<0.01), phospho-PDK1 (P<0.05), and aPKC ζ (P<0.05) and in decreased PEPCK-C (P<0.01) and G6Pase (P<0.01) expression in the lamb. Our findings highlight the sensitivity of the epigenome to maternal nutrition around conception and the need for dietary interventions that maximize metabolic benefits and minimize metabolic costs for the next generation.-Nicholas, L. M., Rattanatray, L., MacLaughlin, S. M., Ozanne, S. E., Kleemann, D. O.,Walker, S. K., Morrison, J. L., Zhang, S., Muhlhausler, B. S., Martin-Gronert, M. S., McMillen, I. C. Differential effects of maternal obesity and weight loss in the periconceptional period on the epigenetic regulation of hepatic insulin-signaling pathways in the offspring.",
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Nicholas, LM, Rattanatray, L, MacLaughlin, SM, Ozanne, SE, Kleemann, DO, Walker, SK, Morrison, JL, Zhang, S, Muhlhäusler, BS, Martin-Gronert, MS & McMillen, IC 2013, 'Differential effects of maternal obesity and weight loss in the periconceptional period on the epigenetic regulation of hepatic insulin-signaling pathways in the offspring', FASEB Journal, vol. 27, no. 9, pp. 3786-3796. https://doi.org/10.1096/fj.13-227918

Differential effects of maternal obesity and weight loss in the periconceptional period on the epigenetic regulation of hepatic insulin-signaling pathways in the offspring. / Nicholas, Lisa M.; Rattanatray, Leewen; MacLaughlin, Severence M.; Ozanne, Susan E.; Kleemann, Dave O.; Walker, Simon K.; Morrison, Janna L.; Zhang, Song; Muhlhäusler, Beverley S.; Martin-Gronert, Malgorzata S.; McMillen, Isabella C.

In: FASEB Journal, Vol. 27, No. 9, 09.2013, p. 3786-3796.

Research output: Contribution to journalArticle

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T1 - Differential effects of maternal obesity and weight loss in the periconceptional period on the epigenetic regulation of hepatic insulin-signaling pathways in the offspring

AU - Nicholas, Lisa M.

AU - Rattanatray, Leewen

AU - MacLaughlin, Severence M.

AU - Ozanne, Susan E.

AU - Kleemann, Dave O.

AU - Walker, Simon K.

AU - Morrison, Janna L.

AU - Zhang, Song

AU - Muhlhäusler, Beverley S.

AU - Martin-Gronert, Malgorzata S.

AU - McMillen, Isabella C.

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N2 - Our aim was to determine the effect of exposure to maternal obesity or to maternal weight loss around conception on the programming of hepatic insulin signaling in the offspring. We used an embryo transfer model in sheep to investigate the effects of exposure to either maternal obesity or to weight loss in normal and obese mothers preceding and for 1 wk after conception on the expression of hepatic insulin-signaling and gluconeogenic factors and key miRNAs involved in insulin signaling in the offspring. We found that exposure to maternal obesity resulted in increased hepatic miR-29b (P<0.05), miR-103 (P<0.01), and miR-107 (P<0.05) expression, a decrease in IR (P<0.05), phopsho- Akt (P<0.01), and phospho-FoxO1 (P<0.01) abundance, and a paradoxical decrease in 11βHSD1 (P<0.05), PEPCK-C (P<0.01), and PEPCK-M (P<0.05) expression in lambs. These changes were ablated by a period of moderate dietary restriction imposed during the periconceptional period. Maternal dietary restriction alone also resulted in decreased abundance of a separate subset of hepatic insulin-signaling molecules, namely, IRS1 (P<0.05), PDK1 (P<0.01), phospho-PDK1 (P<0.05), and aPKC ζ (P<0.05) and in decreased PEPCK-C (P<0.01) and G6Pase (P<0.01) expression in the lamb. Our findings highlight the sensitivity of the epigenome to maternal nutrition around conception and the need for dietary interventions that maximize metabolic benefits and minimize metabolic costs for the next generation.-Nicholas, L. M., Rattanatray, L., MacLaughlin, S. M., Ozanne, S. E., Kleemann, D. O.,Walker, S. K., Morrison, J. L., Zhang, S., Muhlhausler, B. S., Martin-Gronert, M. S., McMillen, I. C. Differential effects of maternal obesity and weight loss in the periconceptional period on the epigenetic regulation of hepatic insulin-signaling pathways in the offspring.

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KW - Diabetes

KW - Embryo

KW - Fetus

KW - MicroRNAs

KW - Pregnancy

KW - Programming

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