Detecting metabolic differences in fetal and adult sheep adipose and skeletal muscle tissues

Jack R.T. Darby, Alexandra Sorvina, Christie A. Bader, Mitchell C. Lock, Jia Yin Soo, Stacey L. Holman, Mike Seed, Tim Kuchel, Douglas A. Brooks, Sally E. Plush, Janna L. Morrison

Research output: Contribution to journalArticle

1 Citation (Scopus)


The primary metabolic pathway required to produce ATP differs as a result of tissue type, developmental stage and substrate availability. We utilized molecular and histological techniques to define the metabolic status in foetal and adult, adipose and skeletal muscle tissues. Redox ratios of these tissues were also determined optically by two-photon microscopy. Adult perirenal adipose tissue had a higher optical redox ratio than fetal perirenal adipose tissue, which aligned with glycolysis being used for ATP production; whereas adult skeletal muscle had a lower optical redox ratio than fetal skeletal muscle, which aligned with oxygen demanding oxidative phosphorylation activity being utilized for ATP production. We have compared traditional molecular and microscopy techniques of metabolic tissue characterization with optical redox ratios to provide a more comprehensive report on the dynamics of tissue metabolism.

Original languageEnglish
Article numbere201960085
JournalJournal of Biophotonics
Issue number3
Publication statusPublished - 1 Mar 2020


  • adipose
  • fetus
  • metabolism
  • redox
  • skeletal muscle
  • two photon microscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Darby, J. R. T., Sorvina, A., Bader, C. A., Lock, M. C., Soo, J. Y., Holman, S. L., ... Morrison, J. L. (2020). Detecting metabolic differences in fetal and adult sheep adipose and skeletal muscle tissues. Journal of Biophotonics, 13(3), [e201960085].