Detecting metabolic differences in fetal and adult sheep adipose and skeletal muscle tissue

Jack R T Darby, Alexandra Sorvina, Christie A Bader, Mitchell C Lock, Jia Yin Soo, Stacey L Holman, Mike Seed, Tim Kuchel, Douglas A Brooks, Sally E Plush, Janna L Morrison

Research output: Contribution to journalArticle

Abstract

The primary metabolic pathway required to produce ATP differs as a result of tissue type, developmental stage and substrate availability. We utilised molecular and histological techniques to define the metabolic status in fetal and adult, adipose and skeletal muscle tissues. Redox ratios of these tissues were also determined optically by two-photon microscopy. Adult perirenal adipose tissue had a higher optical redox ratio than fetal perirenal adipose tissue, which aligned with glycolysis being used for ATP production; whereas adult skeletal muscle had a lower optical redox ratio than fetal skeletal muscle, which aligned with OXPHOS activity being utilised for ATP production. We have compared traditional molecular and microscopy techniques of metabolic tissue characterisation with optical redox ratios to provide a more comprehensive report on the dynamics of tissue metabolism. This article is protected by copyright. All rights reserved.

LanguageEnglish
Pagese2386
JournalJournal of Biophotonics
DOIs
Publication statusE-pub ahead of print - 2 Dec 2019
Externally publishedYes

Cite this

Darby, J. R. T., Sorvina, A., Bader, C. A., Lock, M. C., Soo, J. Y., Holman, S. L., ... Morrison, J. L. (2019). Detecting metabolic differences in fetal and adult sheep adipose and skeletal muscle tissue. Journal of Biophotonics, e2386. https://doi.org/10.1002/jbio.201960085