Delivery and long-term expression of a 135 kb LDLR genomic DNA locus in vivo by hydrodynamic tail vein injection

Olivia C. Hibbitt, Richard P. Harbottle, Simon N. Waddington, Christine A. Bursill, Charles Coutelle, Keith M. Channon, Richard Wade-Martins

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: The delivery of a complete genomic DNA locus in vivo may prove advantageous for complementation gene therapy, especially when physiological regulation of gene expression is desirable. Hydrodynamic tail vein injection has been shown to be a highly efficient means of non-viral delivery of plasmid DNA to the liver. Here, we apply hydrodynamic tail vein injection to deliver and express large genomic DNA inserts >100 kb in vivo. Methods: Firstly, a size series (12-172 kb) of bacterial artificial chromosome (BAC) plasmids, carrying human genomic DNA inserts, episomal retention elements, and the enhanced green fluorescent protein (EGFP) reporter gene, was delivered to mice by hydrodynamic tail vein injection. Secondly, an episomal BAC vector carrying the whole genomic DNA locus of the human low-density lipoprotein receptor (LDLR) gene, and an expression cassette for the LacZ reporter gene, was delivered by the same method. Results: We show that the efficiency of delivery is independent of vector size, when an equal number of plasmid molecules are used. We also show, by LacZ reporter gene analysis, that BAC delivery within the liver is widespread. Finally, BAC-end PCR, RT-PCR and immunohistochemistry demonstrate plasmid retention and long-term expression (4 months) of human LDLR in transfected hepatocytes. Conclusion: This is the first demonstration of somatic delivery and long-term expression of a genomic DNA transgene >100 kb in vivo and shows that hydrodynamic tail vein injection can be used to deliver and express large genomic DNA transgenes in the liver.

LanguageEnglish
Pages488-497
Number of pages10
JournalJournal of Gene Medicine
Volume9
Issue number6
DOIs
Publication statusPublished - 1 Jun 2007
Externally publishedYes

Keywords

  • Bacterial artificial chromosome (BAC)
  • Genomic DNA
  • Hydrodynamic
  • Non-viral gene therapy

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Drug Discovery
  • Genetics(clinical)

Cite this

Hibbitt, O. C., Harbottle, R. P., Waddington, S. N., Bursill, C. A., Coutelle, C., Channon, K. M., & Wade-Martins, R. (2007). Delivery and long-term expression of a 135 kb LDLR genomic DNA locus in vivo by hydrodynamic tail vein injection. Journal of Gene Medicine, 9(6), 488-497. https://doi.org/10.1002/jgm.1041