Deficiency of 6B11+ invariant NK T-cells in celiac disease

Randall H. Grose, Fiona M. Thompson, Adrian G. Cummins

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Immunoregulatory NK T-cells are deficient in certain autoimmune diseases. The purpose of this study was to investigate any deficiency of immunoregulatory NK T-cells in celiac disease. NK T-cells were identified by flow cytometry with 6B11 and Vα24 markers in blood from 18 normal and 12 celiac subjects. Blood mononuclear cells were stimulated with anti-CD3/CD28 antibodies and intracellular cytokines assessed at 4 h in seven normal and eight celiac subjects. Vα24/GAPDH mRNA was quantitated in duodenal biopsies by real time PCR in 17 control and 13 celiac subjects. NK T-cells in celiac subjects were reduced to 30% of those in normal subjects. Intracellular IL-4, IL-10 and IL-13 increased significantly by 33-41% in normal subjects, but did not change in celiac subjects. Vα24/GAPDH mRNA from celiac subjects was reduced to 5% of levels in control subjects. We conclude that immunoregulatory NK T-cells are deficient in celiac disease.

LanguageEnglish
Pages1846-1851
Number of pages6
JournalDigestive Diseases and Sciences
Volume53
Issue number7
DOIs
Publication statusPublished - 1 Jul 2008
Externally publishedYes

Keywords

  • Celiac disease
  • Immunoregulatory NK T-cells

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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