Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers

Joanna S. Hawkes, Dani Louise Bryan, Robert A. Gibson

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Samples of milk (n = 80) and venous blood were collected at 5 weeks postpartum from 82 lactating mothers. Human milk cells and peripheral blood mononuclear cells were isolated and the production of interleukin-1β, interleukin-6, and tumor necrosis factor-α in the absence and presence of lipopolysaccharide was determined by enzyme-linked immunosorbent assay. Human milk cells spontaneously produced significantly less interleukin-1β, interleukin-6, and tumor necrosis factor-α than peripheral blood mononuclear cells in the absence of stimulation. In vitro stimulation of human milk cells with lipopolysaccharide (500 ng/ml) for 24 hr increased cytokine production by approximately 40-50%, whereas peripheral blood mononuclear cells responded to lipopolysaccharide (200 ng/ml) with increased cytokine production of up to 350%. These observations suggest that cells in milk are capable of active involvement in the production of the interleukin-1β, interleukin-6, and tumor necrosis factor-α in the mammary gland and have the capacity to respond to further stimulation after leaving the breast.

LanguageEnglish
Pages338-344
Number of pages7
JournalJournal of Clinical Immunology
Volume22
Issue number6
DOIs
Publication statusPublished - 2002

Keywords

  • Human milk
  • Human milk cells
  • Interleukin-1β
  • Interleukin-6
  • Peripheral blood mononuclear cells
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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