Culture of murine embryonic stem cells

Ivana Barbaric, T. Neil Dear

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Appropriate culture of murine embryonic stem cells is critical to enabling introduced mutations to be passed through the germ line. ES cells must be carefully cultured to ensure that pluripotency is maintained. The feeder cells and foetal calf serum used to culture the cells can significantly influence germ line transmission potential. Additionally, ES cells can be karyotypically unstable, so determining the karyotype of ES cell lines will increase your confidence in the ability of the manipulated cells to contribute to the germ line in chimaeric mice.

Original languageEnglish
Title of host publicationTransgenesis Techniques
Subtitle of host publicationPrinciples and Protocols
EditorsElizabeth Cartwright
Pages161-184
Number of pages24
DOIs
Publication statusPublished - 1 Dec 2009

Publication series

NameMethods in Molecular Biology
Volume561
ISSN (Print)1064-3745

Keywords

  • Feeder cells
  • Freezing
  • Karyotype
  • LIF
  • MEF
  • Thawing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Barbaric, I., & Dear, T. N. (2009). Culture of murine embryonic stem cells. In E. Cartwright (Ed.), Transgenesis Techniques: Principles and Protocols (pp. 161-184). (Methods in Molecular Biology; Vol. 561). https://doi.org/10.1007/978-1-60327-019-9_11