We recently found an error in ourcalculation of the probabilityof a wild-type allele moving froms to j susceptible sites (Psj), and acquiring the gene drive (Psg), during gene-drive homing, under the assumption thatmultiplexed gRNAs are expressed simultaneously. In the R code provided (function GeneDriveSimRec, appendix S1), these probabilities are calculated recursively and the multiplier (1-PN)j was missing (from line 11), where j is the number of cut target sites along an inter-site NHEJ-mediated deletion at which NHEJ did not occur. We have assessed the impact of this omission on our results, and it is negligible for the baseline of PN = 0:02 used in our study. However, the impact of this error increases as PN increases. Therefore, we have redone the sensitivity analysis in which we tested PN ranging up to 0.1. The amended figure 6 below is essentially identical to the original figure, with the exception that probabilities of eradication are slightly lower for PN close to 0.1 in panel (c) of the corrected version.(Figure Persented).This same error invalidates the expression provided in the paper for the probability of successful homing (Psg) for a wild-type allele with s susceptible sites, assuming multiplexed gRNAs are expressed simultaneously. The original expression published was:(Equation Persented).
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
- Environmental Science(all)
- Agricultural and Biological Sciences(all)