Contamination in HPLC quantified benzaldehyde is from polypropylene microtubes

Tomer Shemesh, Connie Karschimkus, Kevin G. Rowley

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Semicarbazide-sensitive amine oxidase (SSAO; EC is a copper-containing enzyme predominantly expressed by vascular smooth muscle cells. SSAO deaminates primary amines to produce aldehydes and oxygen peroxides, and may thus play a role in vascular damage. SSAO activity can be quantified by assaying benzaldehyde production using fluorescent derivatisation and separation by HPLC. We performed the derivatisation step in polypropylene or borosilicate glass tubes over 45 min at 95°C. High and obstructing background levels of benzaldehyde were found in one batch of polypropylene vials, as opposed to its alternatives. Treatment and handling of product shipment into the country did not account for introduction of contaminant into packaged vials nor did any reagent used in the assay. We conclude that the source of contamination was most likely due to variation in the commercial production process. Use of borosilicate vials for assays based on aldehyde production and derivatisation is recommended.

Original languageEnglish
Pages (from-to)277-278
Number of pages2
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Issue number1-2
Publication statusPublished or Issued - 5 Nov 2005
Externally publishedYes


  • Benzaldehyde
  • HPLC
  • Semicarbazide-sensitive amine oxidase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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