Complex ciprofloxacin resistome revealed by screening a Pseudomonas aeruginosa mutant library for altered susceptibility

Elena B.M. Breidenstein, Bhavjinder K. Khaira, Irith Wiegand, Joerg Overhage, Robert E.W. Hancock

Research output: Contribution to journalArticlepeer-review

119 Citations (Scopus)


Pseudomonas aeruginosa offers substantial therapeutic challenges due to its high intrinsic resistance to many antibiotics and its propensity to develop mutational and/or adaptive resistance. The PA14 comprehensive mutant library was screened for mutants exhibiting either two- to eightfold increased susceptibilities (revealing genes involved in intrinsic resistance) or decreased susceptibilities (mutational resistance) to the fluoroquinolone ciprofloxacin. Thirty-five and 79 mutants with increased and decreased susceptibilities, respectively, were identified, as confirmed by broth dilution.

Original languageEnglish
Pages (from-to)4486-4491
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Issue number12
Publication statusPublished or Issued - Dec 2008
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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