Comparison of the compositions of the stool microbiotas of infants fed goat milk formula, cow milk-based formula, or breast milk

Gerald W. Tannock, Blair Lawley, Karen Munro, Siva Gowri Pathmanathan, Shao J. Zhou, Maria Makrides, Robert A. Gibson, Thomas Sullivan, Colin G. Prosser, Dianne Lowry, Alison J. Hodgkinson

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The aim of the study was to compare the compositions of the fecal microbiotas of infants fed goat milk formula to those of infants fed cow milk formula or breast milk as the gold standard. Pyrosequencing of 16S rRNA gene sequences was used in the analysis of the microbiotas in stool samples collected from 90 Australian babies (30 in each group) at 2 months of age. Beta-diversity analysis of total microbiota sequences and Lachnospiraceae sequences revealed that they were more similar in breast milk/goat milk comparisons than in breast milk/cow milk comparisons. The Lachnospiraceae were mostly restricted to a single species (Ruminococcus gnavus) in breast milk-fed and goat milk-fed babies compared to a more diverse collection in cow milkfed babies. Bifidobacteriaceae were abundant in the microbiotas of infants in all three groups. Bifidobacterium longum, Bifidobacterium breve, and Bifidobacterium bifidum were the most commonly detected bifidobacterial species. A semiquantitative PCR method was devised to differentiate between B. longum subsp. longum and B. longum subsp. infantis and was used to test stool samples. B. longum subsp. infantis was seldom present in stools, even of breast milk-fed babies. The presence of B. bifidum in the stools of breast milk-fed infants at abundances greater than 10% of the total microbiota was associated with the highest total abundances of Bifidobacteriaceae. When Bifidobacteriaceae abundance was low, Lachnospiraceae abundances were greater. New information about the composition of the fecal microbiota when goat milk formula is used in infant nutrition was thus obtained.

LanguageEnglish
Pages3040-3048
Number of pages9
JournalApplied and Environmental Microbiology
Volume79
Issue number9
DOIs
Publication statusPublished - 1 May 2013

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Tannock, Gerald W. ; Lawley, Blair ; Munro, Karen ; Pathmanathan, Siva Gowri ; Zhou, Shao J. ; Makrides, Maria ; Gibson, Robert A. ; Sullivan, Thomas ; Prosser, Colin G. ; Lowry, Dianne ; Hodgkinson, Alison J. / Comparison of the compositions of the stool microbiotas of infants fed goat milk formula, cow milk-based formula, or breast milk. In: Applied and Environmental Microbiology. 2013 ; Vol. 79, No. 9. pp. 3040-3048.
@article{2e9eed9560444cb0924731cc65825e32,
title = "Comparison of the compositions of the stool microbiotas of infants fed goat milk formula, cow milk-based formula, or breast milk",
abstract = "The aim of the study was to compare the compositions of the fecal microbiotas of infants fed goat milk formula to those of infants fed cow milk formula or breast milk as the gold standard. Pyrosequencing of 16S rRNA gene sequences was used in the analysis of the microbiotas in stool samples collected from 90 Australian babies (30 in each group) at 2 months of age. Beta-diversity analysis of total microbiota sequences and Lachnospiraceae sequences revealed that they were more similar in breast milk/goat milk comparisons than in breast milk/cow milk comparisons. The Lachnospiraceae were mostly restricted to a single species (Ruminococcus gnavus) in breast milk-fed and goat milk-fed babies compared to a more diverse collection in cow milkfed babies. Bifidobacteriaceae were abundant in the microbiotas of infants in all three groups. Bifidobacterium longum, Bifidobacterium breve, and Bifidobacterium bifidum were the most commonly detected bifidobacterial species. A semiquantitative PCR method was devised to differentiate between B. longum subsp. longum and B. longum subsp. infantis and was used to test stool samples. B. longum subsp. infantis was seldom present in stools, even of breast milk-fed babies. The presence of B. bifidum in the stools of breast milk-fed infants at abundances greater than 10{\%} of the total microbiota was associated with the highest total abundances of Bifidobacteriaceae. When Bifidobacteriaceae abundance was low, Lachnospiraceae abundances were greater. New information about the composition of the fecal microbiota when goat milk formula is used in infant nutrition was thus obtained.",
author = "Tannock, {Gerald W.} and Blair Lawley and Karen Munro and Pathmanathan, {Siva Gowri} and Zhou, {Shao J.} and Maria Makrides and Gibson, {Robert A.} and Thomas Sullivan and Prosser, {Colin G.} and Dianne Lowry and Hodgkinson, {Alison J.}",
year = "2013",
month = "5",
day = "1",
doi = "10.1128/AEM.03910-12",
language = "English",
volume = "79",
pages = "3040--3048",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "9",

}

Comparison of the compositions of the stool microbiotas of infants fed goat milk formula, cow milk-based formula, or breast milk. / Tannock, Gerald W.; Lawley, Blair; Munro, Karen; Pathmanathan, Siva Gowri; Zhou, Shao J.; Makrides, Maria; Gibson, Robert A.; Sullivan, Thomas; Prosser, Colin G.; Lowry, Dianne; Hodgkinson, Alison J.

In: Applied and Environmental Microbiology, Vol. 79, No. 9, 01.05.2013, p. 3040-3048.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparison of the compositions of the stool microbiotas of infants fed goat milk formula, cow milk-based formula, or breast milk

AU - Tannock, Gerald W.

AU - Lawley, Blair

AU - Munro, Karen

AU - Pathmanathan, Siva Gowri

AU - Zhou, Shao J.

AU - Makrides, Maria

AU - Gibson, Robert A.

AU - Sullivan, Thomas

AU - Prosser, Colin G.

AU - Lowry, Dianne

AU - Hodgkinson, Alison J.

PY - 2013/5/1

Y1 - 2013/5/1

N2 - The aim of the study was to compare the compositions of the fecal microbiotas of infants fed goat milk formula to those of infants fed cow milk formula or breast milk as the gold standard. Pyrosequencing of 16S rRNA gene sequences was used in the analysis of the microbiotas in stool samples collected from 90 Australian babies (30 in each group) at 2 months of age. Beta-diversity analysis of total microbiota sequences and Lachnospiraceae sequences revealed that they were more similar in breast milk/goat milk comparisons than in breast milk/cow milk comparisons. The Lachnospiraceae were mostly restricted to a single species (Ruminococcus gnavus) in breast milk-fed and goat milk-fed babies compared to a more diverse collection in cow milkfed babies. Bifidobacteriaceae were abundant in the microbiotas of infants in all three groups. Bifidobacterium longum, Bifidobacterium breve, and Bifidobacterium bifidum were the most commonly detected bifidobacterial species. A semiquantitative PCR method was devised to differentiate between B. longum subsp. longum and B. longum subsp. infantis and was used to test stool samples. B. longum subsp. infantis was seldom present in stools, even of breast milk-fed babies. The presence of B. bifidum in the stools of breast milk-fed infants at abundances greater than 10% of the total microbiota was associated with the highest total abundances of Bifidobacteriaceae. When Bifidobacteriaceae abundance was low, Lachnospiraceae abundances were greater. New information about the composition of the fecal microbiota when goat milk formula is used in infant nutrition was thus obtained.

AB - The aim of the study was to compare the compositions of the fecal microbiotas of infants fed goat milk formula to those of infants fed cow milk formula or breast milk as the gold standard. Pyrosequencing of 16S rRNA gene sequences was used in the analysis of the microbiotas in stool samples collected from 90 Australian babies (30 in each group) at 2 months of age. Beta-diversity analysis of total microbiota sequences and Lachnospiraceae sequences revealed that they were more similar in breast milk/goat milk comparisons than in breast milk/cow milk comparisons. The Lachnospiraceae were mostly restricted to a single species (Ruminococcus gnavus) in breast milk-fed and goat milk-fed babies compared to a more diverse collection in cow milkfed babies. Bifidobacteriaceae were abundant in the microbiotas of infants in all three groups. Bifidobacterium longum, Bifidobacterium breve, and Bifidobacterium bifidum were the most commonly detected bifidobacterial species. A semiquantitative PCR method was devised to differentiate between B. longum subsp. longum and B. longum subsp. infantis and was used to test stool samples. B. longum subsp. infantis was seldom present in stools, even of breast milk-fed babies. The presence of B. bifidum in the stools of breast milk-fed infants at abundances greater than 10% of the total microbiota was associated with the highest total abundances of Bifidobacteriaceae. When Bifidobacteriaceae abundance was low, Lachnospiraceae abundances were greater. New information about the composition of the fecal microbiota when goat milk formula is used in infant nutrition was thus obtained.

UR - http://www.scopus.com/inward/record.url?scp=84876312229&partnerID=8YFLogxK

U2 - 10.1128/AEM.03910-12

DO - 10.1128/AEM.03910-12

M3 - Article

VL - 79

SP - 3040

EP - 3048

JO - Applied and Environmental Microbiology

T2 - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 9

ER -