Comparison of Stain-Free gels with traditional immunoblot loading control methodology

Alex D. Colella, Nusha Chegenii, Melinda N. Tea, Ian L. Gibbins, Keryn A. Williams, Tim K. Chataway

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)

Abstract

Loading controls are necessary for semiquantitative Western blotting to compensate for loading errors. Loading control methods include the reprobing of membranes with an antibody against a constitutively expressed protein or staining the membrane with a total protein stain. We compared the loading control performance of recently released Stain-Free (SF) gels with Sypro Ruby (SR) and reprobing using β-actin. SF gels demonstrated superior performance in that they were faster, required fewer steps and consumables, and allowed the quality of electrophoresis and Western transfer to be assessed before committing to costly and time-consuming Western blots.

Original languageEnglish
Pages (from-to)108-110
Number of pages3
JournalAnalytical Biochemistry
Volume430
Issue number2
DOIs
Publication statusPublished or Issued - 15 Nov 2012
Externally publishedYes

Keywords

  • Immunoblot
  • Loading control
  • Semiquantitative
  • Stain-Free gel
  • Western blot

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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