Comparison of four immunoassays to measure serum ferritin concentrations and iron deficiency prevalence among non-pregnant Cambodian women and Congolese children

Crystal D. Karakochuk, Kyly C. Whitfield, Aviva I. Rappaport, Susan I. Barr, Suzanne M. Vercauteren, Judy McLean, Kroeun Hou, Aminuzzaman Talukder, Lisa A. Houghton, Karl B. Bailey, Erick Boy, Timothy J. Green

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Global standardization of ferritin assays is lacking, which could have direct implications on the accurate measurement and comparability of ferritin concentration and iron deficiency (ID) prevalence rates in at-risk populations. We measured serum ferritin concentrations using four immunoassays: the s-ELISA and the AxSYM™ analyzer were compared among 420 non-pregnant Cambodian women; the Centaur® XP analyzer, s-ELISA, and AxSYM™ analyzer were compared among a subset of 100 Cambodian women; and the s-ELISA and the Elecsys® 2010 analyzer were compared among 226 Congolese children aged 6-59 months. Median ferritin concentrations (adjusted for inflammation) ranged between 48 and 91 μg/L among Cambodian women and between 54 and 55 μg/L among Congolese children. ID prevalence ranged from 2% to 10% among Cambodian women and 5% to 7% among Congolese children. Bias between methods varied widely (-9 to 45 μg/L) among women, and was 43 μg/L among children. Bias was lower when ferritin values outside of the s-ELISA measurement range (>250 μg/L) were excluded. The observed differences in ferritin concentrations likely reflect different ferritin isoforms, antibodies, and calibrators used across assays and by different laboratories. However, despite differences in ferritin concentrations, ID prevalence was relatively similar and low across all methods.

LanguageEnglish
Pages65-72
Number of pages8
JournalClinical Chemistry and Laboratory Medicine
Volume55
Issue number1
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Cambodia
  • Congo
  • bias
  • concordance
  • ferritin
  • traceability

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Karakochuk, Crystal D. ; Whitfield, Kyly C. ; Rappaport, Aviva I. ; Barr, Susan I. ; Vercauteren, Suzanne M. ; McLean, Judy ; Hou, Kroeun ; Talukder, Aminuzzaman ; Houghton, Lisa A. ; Bailey, Karl B. ; Boy, Erick ; Green, Timothy J. / Comparison of four immunoassays to measure serum ferritin concentrations and iron deficiency prevalence among non-pregnant Cambodian women and Congolese children. In: Clinical Chemistry and Laboratory Medicine. 2017 ; Vol. 55, No. 1. pp. 65-72.
@article{57679b42a4ca4ef38be7248b0570caa2,
title = "Comparison of four immunoassays to measure serum ferritin concentrations and iron deficiency prevalence among non-pregnant Cambodian women and Congolese children",
abstract = "Global standardization of ferritin assays is lacking, which could have direct implications on the accurate measurement and comparability of ferritin concentration and iron deficiency (ID) prevalence rates in at-risk populations. We measured serum ferritin concentrations using four immunoassays: the s-ELISA and the AxSYM™ analyzer were compared among 420 non-pregnant Cambodian women; the Centaur{\circledR} XP analyzer, s-ELISA, and AxSYM™ analyzer were compared among a subset of 100 Cambodian women; and the s-ELISA and the Elecsys{\circledR} 2010 analyzer were compared among 226 Congolese children aged 6-59 months. Median ferritin concentrations (adjusted for inflammation) ranged between 48 and 91 μg/L among Cambodian women and between 54 and 55 μg/L among Congolese children. ID prevalence ranged from 2{\%} to 10{\%} among Cambodian women and 5{\%} to 7{\%} among Congolese children. Bias between methods varied widely (-9 to 45 μg/L) among women, and was 43 μg/L among children. Bias was lower when ferritin values outside of the s-ELISA measurement range (>250 μg/L) were excluded. The observed differences in ferritin concentrations likely reflect different ferritin isoforms, antibodies, and calibrators used across assays and by different laboratories. However, despite differences in ferritin concentrations, ID prevalence was relatively similar and low across all methods.",
keywords = "Cambodia, Congo, bias, concordance, ferritin, traceability",
author = "Karakochuk, {Crystal D.} and Whitfield, {Kyly C.} and Rappaport, {Aviva I.} and Barr, {Susan I.} and Vercauteren, {Suzanne M.} and Judy McLean and Kroeun Hou and Aminuzzaman Talukder and Houghton, {Lisa A.} and Bailey, {Karl B.} and Erick Boy and Green, {Timothy J.}",
year = "2017",
month = "1",
day = "1",
doi = "10.1515/cclm-2016-0421",
language = "English",
volume = "55",
pages = "65--72",
journal = "Clinical Chemistry and Laboratory Medicine",
issn = "1434-6621",
publisher = "Walter de Gruyter GmbH",
number = "1",

}

Karakochuk, CD, Whitfield, KC, Rappaport, AI, Barr, SI, Vercauteren, SM, McLean, J, Hou, K, Talukder, A, Houghton, LA, Bailey, KB, Boy, E & Green, TJ 2017, 'Comparison of four immunoassays to measure serum ferritin concentrations and iron deficiency prevalence among non-pregnant Cambodian women and Congolese children', Clinical Chemistry and Laboratory Medicine, vol. 55, no. 1, pp. 65-72. https://doi.org/10.1515/cclm-2016-0421

Comparison of four immunoassays to measure serum ferritin concentrations and iron deficiency prevalence among non-pregnant Cambodian women and Congolese children. / Karakochuk, Crystal D.; Whitfield, Kyly C.; Rappaport, Aviva I.; Barr, Susan I.; Vercauteren, Suzanne M.; McLean, Judy; Hou, Kroeun; Talukder, Aminuzzaman; Houghton, Lisa A.; Bailey, Karl B.; Boy, Erick; Green, Timothy J.

In: Clinical Chemistry and Laboratory Medicine, Vol. 55, No. 1, 01.01.2017, p. 65-72.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparison of four immunoassays to measure serum ferritin concentrations and iron deficiency prevalence among non-pregnant Cambodian women and Congolese children

AU - Karakochuk, Crystal D.

AU - Whitfield, Kyly C.

AU - Rappaport, Aviva I.

AU - Barr, Susan I.

AU - Vercauteren, Suzanne M.

AU - McLean, Judy

AU - Hou, Kroeun

AU - Talukder, Aminuzzaman

AU - Houghton, Lisa A.

AU - Bailey, Karl B.

AU - Boy, Erick

AU - Green, Timothy J.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Global standardization of ferritin assays is lacking, which could have direct implications on the accurate measurement and comparability of ferritin concentration and iron deficiency (ID) prevalence rates in at-risk populations. We measured serum ferritin concentrations using four immunoassays: the s-ELISA and the AxSYM™ analyzer were compared among 420 non-pregnant Cambodian women; the Centaur® XP analyzer, s-ELISA, and AxSYM™ analyzer were compared among a subset of 100 Cambodian women; and the s-ELISA and the Elecsys® 2010 analyzer were compared among 226 Congolese children aged 6-59 months. Median ferritin concentrations (adjusted for inflammation) ranged between 48 and 91 μg/L among Cambodian women and between 54 and 55 μg/L among Congolese children. ID prevalence ranged from 2% to 10% among Cambodian women and 5% to 7% among Congolese children. Bias between methods varied widely (-9 to 45 μg/L) among women, and was 43 μg/L among children. Bias was lower when ferritin values outside of the s-ELISA measurement range (>250 μg/L) were excluded. The observed differences in ferritin concentrations likely reflect different ferritin isoforms, antibodies, and calibrators used across assays and by different laboratories. However, despite differences in ferritin concentrations, ID prevalence was relatively similar and low across all methods.

AB - Global standardization of ferritin assays is lacking, which could have direct implications on the accurate measurement and comparability of ferritin concentration and iron deficiency (ID) prevalence rates in at-risk populations. We measured serum ferritin concentrations using four immunoassays: the s-ELISA and the AxSYM™ analyzer were compared among 420 non-pregnant Cambodian women; the Centaur® XP analyzer, s-ELISA, and AxSYM™ analyzer were compared among a subset of 100 Cambodian women; and the s-ELISA and the Elecsys® 2010 analyzer were compared among 226 Congolese children aged 6-59 months. Median ferritin concentrations (adjusted for inflammation) ranged between 48 and 91 μg/L among Cambodian women and between 54 and 55 μg/L among Congolese children. ID prevalence ranged from 2% to 10% among Cambodian women and 5% to 7% among Congolese children. Bias between methods varied widely (-9 to 45 μg/L) among women, and was 43 μg/L among children. Bias was lower when ferritin values outside of the s-ELISA measurement range (>250 μg/L) were excluded. The observed differences in ferritin concentrations likely reflect different ferritin isoforms, antibodies, and calibrators used across assays and by different laboratories. However, despite differences in ferritin concentrations, ID prevalence was relatively similar and low across all methods.

KW - Cambodia

KW - Congo

KW - bias

KW - concordance

KW - ferritin

KW - traceability

UR - http://www.scopus.com/inward/record.url?scp=84998880897&partnerID=8YFLogxK

U2 - 10.1515/cclm-2016-0421

DO - 10.1515/cclm-2016-0421

M3 - Article

VL - 55

SP - 65

EP - 72

JO - Clinical Chemistry and Laboratory Medicine

T2 - Clinical Chemistry and Laboratory Medicine

JF - Clinical Chemistry and Laboratory Medicine

SN - 1434-6621

IS - 1

ER -