Cloning of cDNA for the β-subunit of rabbit translation initiation factor-2 using PCR

Nigel T. Price, Len Hall, Christopher Proud

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. Rabbit eIF-2β transcripts were then specifically amplified by PCR and sequenced. RACE (rapid amplification of cDNA ends) was used to obtain 3′ and 5′ sequences. Comparison of the deduced amino acid sequence with that of human eIF-2β reveals a very high degree of sequence identity.

Original languageEnglish
Pages (from-to)170-172
Number of pages3
JournalBBA - Gene Structure and Expression
Volume1216
Issue number1
DOIs
Publication statusPublished or Issued - 19 Oct 1993
Externally publishedYes

Keywords

  • (Rabbit)
  • Eukaryote
  • Initiation factor-2
  • PCR
  • Protein synthesis
  • cDNA cloning

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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