Cloning and sequence analysis of caprine N-acetylglucosamine 6-sulfatase cDNA

Karen Friderici, Kevin T. Cavanagh, Jeffrey R. Leipprandt, Christine E. Traviss, Donald S. Anson, John J. Hopwood, Margaret Z. Jones

Research output: Contribution to journalArticle

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Abstract

Mucopolysaccharidosis BID results from the deficiency of N-acetylglucosamine 6-sulfatase activity. A Nubian goat with this lysosomal storage disease has been identified. As a first step in developing this animal model for testing treatment methods, we cloned and sequenced the capfne N-acetylglucosamine 6-sulfatase cDNA coding region. Overall there is 88% nucleotide homology between the goat and human sequence and 94% homology of the deduced amino acid sequence. The human and two ruminant species differ by the presence of an imperfect trinucleotide (CCG) repeat in the ruminant signal sequence.

Original languageEnglish
Pages (from-to)369-373
Number of pages5
JournalBBA - Molecular Basis of Disease
Volume1271
Issue number2-3
DOIs
Publication statusPublished - 9 Jun 1995

Keywords

  • (Human)
  • Caprine
  • Comparative genomics
  • Mucopolysaccharidosis IIID
  • N-Acetylglucosamine 6-sulfatase
  • Trinucleetide repeat
  • cDNA

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology

Cite this

Friderici, K., Cavanagh, K. T., Leipprandt, J. R., Traviss, C. E., Anson, D. S., Hopwood, J. J., & Jones, M. Z. (1995). Cloning and sequence analysis of caprine N-acetylglucosamine 6-sulfatase cDNA. BBA - Molecular Basis of Disease, 1271(2-3), 369-373. https://doi.org/10.1016/0925-4439(95)00054-8