Cloning and characterization of a new human Xq13 gene, encoding a putative helicase

Carol L. Stayton, Branka Dabovic, Massimo Gulisano, Jozef Gecz, Vania Broccoll, Serenella Glovanazzl, Michela Bossolasco, Lucia Monaco, Sohalla Rastan, Edoardo Boncinelli, Marco E. Blanchil, G. Giacomo Consalez

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We describe the cloning and characterization of a new human Xq13 gene (XH2), extending over a 220 kb genomlc stretch between MNK and DXS56. The gene, which undergoes X-inactivatlon, contains a 4 kb open reading frame and encodes a putative NTP-blndlng nuclear protein homologous to several members of the helicase II superfamily. The murine homologue maps to the syntenlc genetic interval, between Pgk1 and Xlst. In situ hybridization studies In mouse reveal precocious, widespread expression of the murine homologue of XH2 at early stages of embryogenesis, and more restricted expression during late developmental stages and at birth. XH2 Is a new member of an expanding family of proven and putative helicases, sharing six conserved, colllnear domains. In particular, the XH2 protein shows homology with yeast RAD54. Type II helicases have been Implicated in nucleotide excision repair and the initiation of transcription. This new gene, represents a potential candidate for several genetic disorders mapped to human Xq13.

LanguageEnglish
Pages1957-1964
Number of pages8
JournalHuman Molecular Genetics
Volume3
Issue number11
DOIs
Publication statusPublished - 1 Nov 1994
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Stayton, C. L., Dabovic, B., Gulisano, M., Gecz, J., Broccoll, V., Glovanazzl, S., ... Consalez, G. G. (1994). Cloning and characterization of a new human Xq13 gene, encoding a putative helicase. Human Molecular Genetics, 3(11), 1957-1964. https://doi.org/10.1093/hmg/3.11.1957
Stayton, Carol L. ; Dabovic, Branka ; Gulisano, Massimo ; Gecz, Jozef ; Broccoll, Vania ; Glovanazzl, Serenella ; Bossolasco, Michela ; Monaco, Lucia ; Rastan, Sohalla ; Boncinelli, Edoardo ; Blanchil, Marco E. ; Consalez, G. Giacomo. / Cloning and characterization of a new human Xq13 gene, encoding a putative helicase. In: Human Molecular Genetics. 1994 ; Vol. 3, No. 11. pp. 1957-1964.
@article{4d61f0eea05e44f8932c2d55e0f0f02b,
title = "Cloning and characterization of a new human Xq13 gene, encoding a putative helicase",
abstract = "We describe the cloning and characterization of a new human Xq13 gene (XH2), extending over a 220 kb genomlc stretch between MNK and DXS56. The gene, which undergoes X-inactivatlon, contains a 4 kb open reading frame and encodes a putative NTP-blndlng nuclear protein homologous to several members of the helicase II superfamily. The murine homologue maps to the syntenlc genetic interval, between Pgk1 and Xlst. In situ hybridization studies In mouse reveal precocious, widespread expression of the murine homologue of XH2 at early stages of embryogenesis, and more restricted expression during late developmental stages and at birth. XH2 Is a new member of an expanding family of proven and putative helicases, sharing six conserved, colllnear domains. In particular, the XH2 protein shows homology with yeast RAD54. Type II helicases have been Implicated in nucleotide excision repair and the initiation of transcription. This new gene, represents a potential candidate for several genetic disorders mapped to human Xq13.",
author = "Stayton, {Carol L.} and Branka Dabovic and Massimo Gulisano and Jozef Gecz and Vania Broccoll and Serenella Glovanazzl and Michela Bossolasco and Lucia Monaco and Sohalla Rastan and Edoardo Boncinelli and Blanchil, {Marco E.} and Consalez, {G. Giacomo}",
year = "1994",
month = "11",
day = "1",
doi = "10.1093/hmg/3.11.1957",
language = "English",
volume = "3",
pages = "1957--1964",
journal = "Human molecular genetics",
issn = "0964-6906",
publisher = "Oxford University Press",
number = "11",

}

Stayton, CL, Dabovic, B, Gulisano, M, Gecz, J, Broccoll, V, Glovanazzl, S, Bossolasco, M, Monaco, L, Rastan, S, Boncinelli, E, Blanchil, ME & Consalez, GG 1994, 'Cloning and characterization of a new human Xq13 gene, encoding a putative helicase', Human Molecular Genetics, vol. 3, no. 11, pp. 1957-1964. https://doi.org/10.1093/hmg/3.11.1957

Cloning and characterization of a new human Xq13 gene, encoding a putative helicase. / Stayton, Carol L.; Dabovic, Branka; Gulisano, Massimo; Gecz, Jozef; Broccoll, Vania; Glovanazzl, Serenella; Bossolasco, Michela; Monaco, Lucia; Rastan, Sohalla; Boncinelli, Edoardo; Blanchil, Marco E.; Consalez, G. Giacomo.

In: Human Molecular Genetics, Vol. 3, No. 11, 01.11.1994, p. 1957-1964.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cloning and characterization of a new human Xq13 gene, encoding a putative helicase

AU - Stayton, Carol L.

AU - Dabovic, Branka

AU - Gulisano, Massimo

AU - Gecz, Jozef

AU - Broccoll, Vania

AU - Glovanazzl, Serenella

AU - Bossolasco, Michela

AU - Monaco, Lucia

AU - Rastan, Sohalla

AU - Boncinelli, Edoardo

AU - Blanchil, Marco E.

AU - Consalez, G. Giacomo

PY - 1994/11/1

Y1 - 1994/11/1

N2 - We describe the cloning and characterization of a new human Xq13 gene (XH2), extending over a 220 kb genomlc stretch between MNK and DXS56. The gene, which undergoes X-inactivatlon, contains a 4 kb open reading frame and encodes a putative NTP-blndlng nuclear protein homologous to several members of the helicase II superfamily. The murine homologue maps to the syntenlc genetic interval, between Pgk1 and Xlst. In situ hybridization studies In mouse reveal precocious, widespread expression of the murine homologue of XH2 at early stages of embryogenesis, and more restricted expression during late developmental stages and at birth. XH2 Is a new member of an expanding family of proven and putative helicases, sharing six conserved, colllnear domains. In particular, the XH2 protein shows homology with yeast RAD54. Type II helicases have been Implicated in nucleotide excision repair and the initiation of transcription. This new gene, represents a potential candidate for several genetic disorders mapped to human Xq13.

AB - We describe the cloning and characterization of a new human Xq13 gene (XH2), extending over a 220 kb genomlc stretch between MNK and DXS56. The gene, which undergoes X-inactivatlon, contains a 4 kb open reading frame and encodes a putative NTP-blndlng nuclear protein homologous to several members of the helicase II superfamily. The murine homologue maps to the syntenlc genetic interval, between Pgk1 and Xlst. In situ hybridization studies In mouse reveal precocious, widespread expression of the murine homologue of XH2 at early stages of embryogenesis, and more restricted expression during late developmental stages and at birth. XH2 Is a new member of an expanding family of proven and putative helicases, sharing six conserved, colllnear domains. In particular, the XH2 protein shows homology with yeast RAD54. Type II helicases have been Implicated in nucleotide excision repair and the initiation of transcription. This new gene, represents a potential candidate for several genetic disorders mapped to human Xq13.

UR - http://www.scopus.com/inward/record.url?scp=0028114291&partnerID=8YFLogxK

U2 - 10.1093/hmg/3.11.1957

DO - 10.1093/hmg/3.11.1957

M3 - Article

VL - 3

SP - 1957

EP - 1964

JO - Human molecular genetics

T2 - Human molecular genetics

JF - Human molecular genetics

SN - 0964-6906

IS - 11

ER -