Class I and II histone deacetylase expression in human chronic periodontitis gingival tissue

M. D. Cantley, A. A.S.S.K. Dharmapatni, K. Algate, T. N. Crotti, P. M. Bartold, D. R. Haynes

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Background and Objective: Histone deacetylase inhibitors (HDACi) are being considered to treat chronic inflammatory diseases at low doses. Currently HDACi that are more specific are being developed to target particular HDACs; therefore, this study aimed to determine levels and distribution of class I and II HDAC in human gingival samples obtained from patients with chronic periodontitis. Material and Methods: Gingival biopsies were obtained from patients with and without (mild inflammation, no bone loss) periodontitis. Total RNA was isolated for real-time quantitative polymerase chain reaction to determine expression of HDACs 1-10. Immunohistochemistry was used to determine protein distribution of HDACs 1, 5, 8 and 9. Factor VIII, CD3 and tartrate resistant acid phosphatase (TRAP) were detected in serial sections to identify blood vessels, lymphocytes, pre-osteoclasts and osteoclasts cells respectively. Tumour necrosis factor α (TNF-α) expression was also assessed. Results: mRNA for HDAC 1, 5, 8 and 9 were significantly upregulated in chronic periodontitis gingival tissues compared to non-periodontitis samples (p < 0.05). Significantly higher HDAC 1 protein expression was observed in chronic periodontitis samples (p < 0.05), and was associated with CD3, TRAP and TNF-α-positive cells. HDAC 1, 5, 8 and 9 were expressed strongly by the factor VIII-positive microvasculature in the chronic periodontitis gingival tissues. Conclusions: HDAC 1, 5, 8 and 9 expression was higher in gingival tissues from patients with chronic periodontitis compared to non-periodontitis samples. Results suggest that these HDACs could therefore be targeted with specific acting HDACi.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalJournal of Periodontal Research
Volume51
Issue number2
DOIs
Publication statusPublished or Issued - 1 Apr 2016
Externally publishedYes

Keywords

  • Histone deacetylases
  • Immunohistochemistry
  • Inflammation
  • Periodontitis

ASJC Scopus subject areas

  • Periodontics

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