Characterization of the EphA1 receptor tyrosine kinase: Expression in epithelial tissues

M. G. Coulthard, J. D. Lickliter, N. Subanesan, K. Chen, G. C. Webb, A. J. Lowry, S. Koblar, C. D K Bottema, A. W. Boyd

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

The Eph family of receptor tyrosine kinases plays a crucial role during development and is implicated in oncogenesis. Using a partial cDNA clone of an Eph-related kinase (Esk) we isolated the complete coding region of a gene which we show to be murine EphA1 by both structural and functional criteria. The chromosomal localization is shown to be syntenic to hEphA1 and the genomic organization also shows distinct features found in the hEphA1 gene. Functionally, in keeping with findings for the human homologue, both soluble recombinant and "native" mEphA1 show preferential binding to ephrin A1. However, we also observed significant binding to other A-type ligands as has been observed for other Eph receptors. We analysed the expression of mEphA1 mRNA by in situ hybridization on tissue sections. mEphA1 was expressed in epithelial elements of skin, adult thymus, kidney and adrenal cortex. Taken together with previous Northern blotting data these results suggest that mEphA1 is expressed widely in differentiated epithelial cells.

Original languageEnglish
Pages (from-to)303-317
Number of pages15
JournalGrowth Factors
Volume18
Issue number4
DOIs
Publication statusPublished or Issued - 2001

Keywords

  • Eph
  • Epithelial tissue
  • Genomic organisation
  • Receptor tyrosine kinase
  • Thymus

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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