Challenges of "sticky" co-immunoprecipitation: Polyalanine tract protein-protein interactions

T. R. Mattiske, May H. Tan, Jozef Gécz, Cheryl Shoubridge

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Citations (Scopus)

Abstract

Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein-protein interactions. Investigating interactions of proteins containing "sticky" polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.

Original languageEnglish
Title of host publicationTandem Repeats in Genes, Proteins, and Disease
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages121-133
Number of pages13
ISBN (Print)9781627034371
DOIs
Publication statusPublished or Issued - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1017
ISSN (Print)1064-3745

Keywords

  • ARX
  • Co-immunoprecipitation
  • Polyalanine tract
  • Protein-protein interaction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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