Cellulases (CM Cases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost

Lungisa Mayende, Brendan S. Wilhelmi, Brett I. Pletschke

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56 Citations (Scopus)

Abstract

In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 °C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 °C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml -1 min-1. Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml-1 min-1. Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis.

Original languageEnglish
Pages (from-to)2963-2966
Number of pages4
JournalSoil Biology and Biochemistry
Volume38
Issue number9
DOIs
Publication statusPublished or Issued - Sep 2006

Keywords

  • Cellulases
  • Compost
  • Polyphenol oxidases
  • Thermophiles

ASJC Scopus subject areas

  • Microbiology
  • Soil Science

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