Biostimulation of indigenous communities for the successful dechlorination of tetrachloroethene (perchloroethylene)-contaminated groundwater

Sayali S. Patil, Eric M. Adetutu, Arturo Aburto-Medina, Ian R. Menz, Andrew S. Ball

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Chlorinated ethenes are of environmental concern with most reports of successful microbial-mediated remediation being associated with major dechlorinating groups such as Dehalococcoides (Dhc) species. However, limited information is available on the community dynamics and dechlorinating activities of indigenous non-Dhc groups. Here, we present evidence of dechlorination of tetrachloroethene (perchloroethylene, PCE) in groundwater samples by indigenous microbial communities. 100 % PCE conversion to ethene was observed in acetate-stimulated 24 week-microcosms (controls; 15 %). Microbial community profiles showed dominance by groups such as Proteobacteria, Spirochaetes, Firmicutes, Methanomicrobiaceae and Methanosarcinaceae. Pareto-Lorenz (PL) analyses suggested an adapted (45 % PL value) but variable bacterial community (55.5 % Δt(week)) compared to Archaea (25 % PL value; 46.9 % Δt(week)). Our findings provide evidence of dechlorinating potential of indigenous microorganisms and useful information on their dynamics which may be exploited for in situ groundwater bioremediation.

Original languageEnglish
Pages (from-to)75-83
Number of pages9
JournalBiotechnology Letters
Volume36
Issue number1
DOIs
Publication statusPublished or Issued - Jan 2014
Externally publishedYes

Keywords

  • Dechlorinating microbial community
  • Groundwater
  • PCR-DGGE
  • Perchloroethylene
  • Reductive dechlorination
  • Tetrachloroethene

ASJC Scopus subject areas

  • Biotechnology

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