Biomineralization of platinum by escherichia coli

Sahar S. Shar, Frank Reith, Esmaeil Shahsavari, Eric Adetutu, Yuana Nurulita, Khalid Al-Hothaly, Nagalakshmi Haleyur, Andrew S. Ball

Research output: Contribution to journalArticle

Abstract

The widespread use of platinum in many industrial applications has led to its release into the environment at elevated concentrations with potential adverse effects on human and environmental health. However, the nature of interactions between mobile platinum complexes and the biotic components of the environment, which are increasingly being exposed to platinum, is poorly studied. The aim of this study was to assess the impact of Pt(IV)-chloride on the growth and activity of the well-characterized bacteria Escherichia coli. Bacterial survival and viability in the presence of different concentrations of Pt(IV)-chloride were assessed in liquid culture, while platinum retention was assessed using experimentation with sand-filled columns with the residual platinum concentration measured by atomic absorption spectroscopy. Bacterial biomineralization of platinum was studied with scanning electron microscopy. The results showed that E. coli tolerated PtCl4 at concentrations of up to 10,000 μM over 21 days and remained viable after 112 days of incubation with PtCl4 at 10,000 μM in sand columns. Overall, 74 wt.% and 50 wt.% of platinum was mineralized in E. coli and blank sand columns, respectively. The results of this study confirm that E. coli is capable of biomineralizing platinum. The results confirm that the interaction of platinum with bacteria is not limited to known metal-resistant bacterial species.

LanguageEnglish
Article number407
JournalMetals
Volume9
Issue number4
DOIs
Publication statusPublished - 1 Apr 2019
Externally publishedYes

Keywords

  • Atomic absorption spectroscopy
  • Cell viability
  • E. coli
  • Platinum
  • Platinum
  • Sand-filled columns
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Shar, S. S., Reith, F., Shahsavari, E., Adetutu, E., Nurulita, Y., Al-Hothaly, K., ... Ball, A. S. (2019). Biomineralization of platinum by escherichia coli. Metals, 9(4), [407]. https://doi.org/10.3390/met9040407
Shar, Sahar S. ; Reith, Frank ; Shahsavari, Esmaeil ; Adetutu, Eric ; Nurulita, Yuana ; Al-Hothaly, Khalid ; Haleyur, Nagalakshmi ; Ball, Andrew S. / Biomineralization of platinum by escherichia coli. In: Metals. 2019 ; Vol. 9, No. 4.
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Shar, SS, Reith, F, Shahsavari, E, Adetutu, E, Nurulita, Y, Al-Hothaly, K, Haleyur, N & Ball, AS 2019, 'Biomineralization of platinum by escherichia coli', Metals, vol. 9, no. 4, 407. https://doi.org/10.3390/met9040407

Biomineralization of platinum by escherichia coli. / Shar, Sahar S.; Reith, Frank; Shahsavari, Esmaeil; Adetutu, Eric; Nurulita, Yuana; Al-Hothaly, Khalid; Haleyur, Nagalakshmi; Ball, Andrew S.

In: Metals, Vol. 9, No. 4, 407, 01.04.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biomineralization of platinum by escherichia coli

AU - Shar, Sahar S.

AU - Reith, Frank

AU - Shahsavari, Esmaeil

AU - Adetutu, Eric

AU - Nurulita, Yuana

AU - Al-Hothaly, Khalid

AU - Haleyur, Nagalakshmi

AU - Ball, Andrew S.

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Shar SS, Reith F, Shahsavari E, Adetutu E, Nurulita Y, Al-Hothaly K et al. Biomineralization of platinum by escherichia coli. Metals. 2019 Apr 1;9(4). 407. https://doi.org/10.3390/met9040407