Bacterial biofilms of importance to medicine and bioterrorism: Proteomic techniques to identify novel vaccine components and drug targets

Daniel J. Hassett, Patrick A. Limbach, Robert F. Hennigan, Karl E. Klose, Robert Hancock, Mark D. Platt, Donald F. Hunt

Research output: Contribution to journalReview article

11 Citations (Scopus)

Abstract

Biofilms are highly ordered microbial communities enmeshed in a carefully sculpted matrix designed for survival of organisms either in multi- or monogenus/species in a specific microniche. In human disease, biofilm infections are some of the most recalcitrant to treat. Even with rigorous antibiotic regimens, some biofilms, such as those within the thick airway mucus of cystic fibrosis (CF) patients, persist throughout the course of the disease process. In this editorial, discussion will cover the utility of using advanced proteomic techniques to help identify potential weaknesses in the already impressive defensive armamentarium of biofilm bacteria. Two biofilm systems will be discussed herein, one of which is that of Pseudomonas aeruginosa biofilms within CF airway biofilms. The other is referred to as persistent 'bioterrorist agent biofilms' in which Francisella tularensis can grow on surfaces where environmental amoeba can phagocytose them, allowing for growth of F. tularensis within the amoebae.

LanguageEnglish
Pages1201-1207
Number of pages7
JournalExpert Opinion on Biological Therapy
Volume3
Issue number8
DOIs
Publication statusPublished - 1 Dec 2003

Keywords

  • Biofilms
  • Francisella tularensis
  • Mass spectrometry
  • Proteomics
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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