Augmentation of the human monocyte/macrophage chemiluminescence response during short-term exposure to interferon-gamma and tumour necrosis factor-alpha

L. M. Kumaratilake, A. Ferrante, E. J. Bates, I. C. Kowanko

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effects of short-term (30 min) pre-incubation of human monocytes and macrophages (3-day cultured monocytes) with leucocyte-derived human interferon-gamma (IFN-γ) and recombinant human tumour necrosis factor-alpha (rTNF-α) were examined. Pre-incubation of either monocytes or macrophages with rTNF-α or IFN-γ (100 U/5 x 105 cells) augmented their respiratory burst to formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), measured by the luminol- and lucigenin-dependent chemiluminescence assay. In addition, both cell types showed a burst of respiratory activity in the presence of rTNF-α or IFN-γ only. The effects of IFN-γ were removed by adsorption with an anti-IFN-γ monoclonal antibody and those of rTNF-α were abolished by heating at 100°C, or by the addition of anti-TNF-α monoclonal antibody. The results demonstrate that both IFN-γ and rTNF-α are stimulators of monocytes and macrophages, and rapidly alter the capacity of the cells to respond to fMLP, which binds to cell surface receptors.

LanguageEnglish
Pages257-262
Number of pages6
JournalClinical and Experimental Immunology
Volume80
Issue number2
Publication statusPublished - 1990
Externally publishedYes

Keywords

  • Chemiluminescence
  • Interferon-gamma
  • Macrophages
  • Monocytes
  • Tumour necrosis factor

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Augmentation of the human monocyte/macrophage chemiluminescence response during short-term exposure to interferon-gamma and tumour necrosis factor-alpha",
abstract = "The effects of short-term (30 min) pre-incubation of human monocytes and macrophages (3-day cultured monocytes) with leucocyte-derived human interferon-gamma (IFN-γ) and recombinant human tumour necrosis factor-alpha (rTNF-α) were examined. Pre-incubation of either monocytes or macrophages with rTNF-α or IFN-γ (100 U/5 x 105 cells) augmented their respiratory burst to formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), measured by the luminol- and lucigenin-dependent chemiluminescence assay. In addition, both cell types showed a burst of respiratory activity in the presence of rTNF-α or IFN-γ only. The effects of IFN-γ were removed by adsorption with an anti-IFN-γ monoclonal antibody and those of rTNF-α were abolished by heating at 100°C, or by the addition of anti-TNF-α monoclonal antibody. The results demonstrate that both IFN-γ and rTNF-α are stimulators of monocytes and macrophages, and rapidly alter the capacity of the cells to respond to fMLP, which binds to cell surface receptors.",
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Augmentation of the human monocyte/macrophage chemiluminescence response during short-term exposure to interferon-gamma and tumour necrosis factor-alpha. / Kumaratilake, L. M.; Ferrante, A.; Bates, E. J.; Kowanko, I. C.

In: Clinical and Experimental Immunology, Vol. 80, No. 2, 1990, p. 257-262.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Ferrante, A.

AU - Bates, E. J.

AU - Kowanko, I. C.

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AB - The effects of short-term (30 min) pre-incubation of human monocytes and macrophages (3-day cultured monocytes) with leucocyte-derived human interferon-gamma (IFN-γ) and recombinant human tumour necrosis factor-alpha (rTNF-α) were examined. Pre-incubation of either monocytes or macrophages with rTNF-α or IFN-γ (100 U/5 x 105 cells) augmented their respiratory burst to formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), measured by the luminol- and lucigenin-dependent chemiluminescence assay. In addition, both cell types showed a burst of respiratory activity in the presence of rTNF-α or IFN-γ only. The effects of IFN-γ were removed by adsorption with an anti-IFN-γ monoclonal antibody and those of rTNF-α were abolished by heating at 100°C, or by the addition of anti-TNF-α monoclonal antibody. The results demonstrate that both IFN-γ and rTNF-α are stimulators of monocytes and macrophages, and rapidly alter the capacity of the cells to respond to fMLP, which binds to cell surface receptors.

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