ATP-dependent copper transport by the Menkes protein in membrane vesicles isolated from cultured Chinese hamster ovary cells

I Voskoboinik, H Brooks, S Smith, P Shen, J Camakaris

Research output: Contribution to journalArticlepeer-review

Abstract

The Menkes (MNK) protein is a vital component of copper homeostasis in mammalian cells. In this paper we provide the first biochemical evidence that the MNK protein functions as a copper-translocating P-type ATPase in mammalian cells. The enzyme activity in membrane vesicles prepared from Chinese hamster ovary cells overexpressing MNK was ATP-dependent, correlated with the amount of MNK and followed Michaelis-Menten kinetics with respect to copper. The copper transport was observed only under reducing conditions suggesting MNK transports Cu(I). This study opens the way to detailed structure-function studies and assessment of functional MNK derived from patients with Menkes disease.

Original languageEnglish
Pages (from-to)178-82
Number of pages5
JournalFEBS Letters
Volume435
Issue number2-3
DOIs
Publication statusPublished or Issued - 18 Sep 1998

Keywords

  • Adenosine Triphosphatases/metabolism
  • Adenosine Triphosphate/metabolism
  • Animals
  • CHO Cells
  • Carrier Proteins/metabolism
  • Cation Transport Proteins
  • Cell Membrane/metabolism
  • Copper/metabolism
  • Cricetinae
  • Cytoplasmic Granules/metabolism
  • Ion Transport
  • Recombinant Fusion Proteins

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