Antimicrobial peptide delivery from trabecular bone grafts

Mehdi Kazemzadeh-Narbat, Qiong Wang, Robert Hancock, Rizhi Wang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Autografts and allografts are extensively used for reconstruction of skeletal defects caused by trauma, tumor resection, and other diseases. They are also often used together with total hip and total knee implants. Similar to synthetic implants, these bone grafts also face the risk of infection. In this report, we propose to combine bone autografting and allografting with local delivery of antimicrobial agents to combat peri-implant infections. A special group of antimicrobial peptides was selected for this purpose to address the concern of developing antibiotic resistance. Both natural and sintered trabecular bone specimens were impregnated with a short cationic antimicrobial peptide HHC-36 (KRWWKWWRR). Antimicrobial activity using a survival assay against Gram-positive bacterium, S. aureus confirmed that the natural bone scaffolds could kill 100% of bacterial cells, while the sintered scaffolds could reduce the number of bacterial cells by 99% within four hours of culture. Such a difference is attributed to the peptide loading efficiency in the natural bone scaffolds which is five times as high as that in the sintered trabecular bone specimens.

Original languageEnglish
Pages (from-to)967-972
Number of pages6
JournalJournal of Biomaterials and Tissue Engineering
Volume4
Issue number11
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Keywords

  • Allografts
  • Antimicrobial Peptides
  • Bone Scaffolds
  • Drug Delivery

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this