Antigen‐specific B cells in tissues after oral typhoid vaccination

C. E. GIBSON, I. A. PENTTILA, A. S‐Y LEONG, H. ZOLA, J. T. LABROOY

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2 Citations (Scopus)

Abstract

Six human subjects who were to receive elective bowel surgery for a variety of diseases were vaccinated with the oral typhoid vaccine, Ty21a. Intestinal tissue (ileum in two, large intestine in four) removed 7‐26 days after the first dose of vaccine was examined for the presence and distribution of antigen‐specific B cells. This was compared with intestinal tissue derived from two unvaccinated controls. A number of B cell differentiation antigens were also assessed on these cells by immunofluorescence using dual‐labelling. Antigen‐specific cells were found randomly distributed in the lamina propria of all the vaccinated subjects in low frequency (6 ± 0.5 to 37 ± 31 [mean ± s.e.m.] antigen specific cells/10 mm2 of tissue). The lymphocyte differentiation antigens CD45RA, CD45RO. L‐selectin, CD‐1 la CD‐38, CD‐44 and VLA‐4 were all found on antigen‐specific cells, but no particular pattern was recognizable in this small series of six subjects with different disease processes affecting the intestine.

Original languageEnglish
Pages (from-to)62-65
Number of pages4
JournalImmunology and Cell Biology
Volume73
Issue number1
DOIs
Publication statusPublished - 1 Jan 1995
Externally publishedYes

Keywords

  • Key words: antigen‐specific B cells
  • human intestinal tissue
  • oral vaccination

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Cite this

GIBSON, C. E., PENTTILA, I. A., LEONG, A. SY., ZOLA, H., & LABROOY, J. T. (1995). Antigen‐specific B cells in tissues after oral typhoid vaccination. Immunology and Cell Biology, 73(1), 62-65. https://doi.org/10.1111/j.1440-1711.1995.tb03835.x