Analysis of the involuting mouse mammary gland: An in vivo model for cell death

Bethan Lloyd-Lewis, Timothy Sargeant, Peter A. Kreuzaler, Henrike K. Resemann, Sara Pensa, Christine J. Watson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Involution of the mammary gland occurs at the end of every period of lactation and is an essential process to return the gland to a pre-pregnant state in readiness for the next pregnancy. Involution is a complex process of regulated alveolar cell death coupled with tissue remodeling and requires exquisite control of transcription and signaling. These processes can be investigated using a variety of molecular and morphological approaches. In this chapter we describe how to initiate involution and collect mammary glands, measure involution morphologically, and quantify lysosomal leakiness in mammary tissue and in cultured mammary epithelial cells. These procedures encompass a range of microscopy and molecular biology techniques.

LanguageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages165-186
Number of pages22
DOIs
Publication statusPublished - 1 Jan 2017

Publication series

NameMethods in Molecular Biology
Volume1501
ISSN (Print)1064-3745

Keywords

  • Caspase 3
  • Cell death
  • Electron microscopy
  • Immunofluorescence
  • Involution
  • Lysosome
  • Mammary gland

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Lloyd-Lewis, B., Sargeant, T., Kreuzaler, P. A., Resemann, H. K., Pensa, S., & Watson, C. J. (2017). Analysis of the involuting mouse mammary gland: An in vivo model for cell death. In Methods in Molecular Biology (pp. 165-186). (Methods in Molecular Biology; Vol. 1501). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6475-8_7
Lloyd-Lewis, Bethan ; Sargeant, Timothy ; Kreuzaler, Peter A. ; Resemann, Henrike K. ; Pensa, Sara ; Watson, Christine J. / Analysis of the involuting mouse mammary gland : An in vivo model for cell death. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 165-186 (Methods in Molecular Biology).
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Lloyd-Lewis, B, Sargeant, T, Kreuzaler, PA, Resemann, HK, Pensa, S & Watson, CJ 2017, Analysis of the involuting mouse mammary gland: An in vivo model for cell death. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1501, Humana Press Inc., pp. 165-186. https://doi.org/10.1007/978-1-4939-6475-8_7

Analysis of the involuting mouse mammary gland : An in vivo model for cell death. / Lloyd-Lewis, Bethan; Sargeant, Timothy; Kreuzaler, Peter A.; Resemann, Henrike K.; Pensa, Sara; Watson, Christine J.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 165-186 (Methods in Molecular Biology; Vol. 1501).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Lloyd-Lewis B, Sargeant T, Kreuzaler PA, Resemann HK, Pensa S, Watson CJ. Analysis of the involuting mouse mammary gland: An in vivo model for cell death. In Methods in Molecular Biology. Humana Press Inc. 2017. p. 165-186. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6475-8_7