An Arginine-Histone Methyltransferase, CARMER, Coordinates Ecdysone-mediated Apoptosis in Drosophila Cells

Dimitrios Cakouros, Tasman J. Daish, Kathryn Mills, Sharad Kumar

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Developmentally programmed cell death is regulated by a balance between pro- and anti-death signaling. During Drosophila metamorphosis, the removal of larval tissues is dependent on the steroid hormone ecdys one, which controls the levels of pro- and anti-death molecules. Ecdysone binds to its heterodimeric receptor ecdysone receptor/ultraspiracle to mediate transcription of primary response genes. Here we show that CARMER, an arginine-histone methyltransferase, is critical in coordinating ecdysone-induced expression of Drosophila cell death genes. Ablation of CARMER blocks ecdysone-induced cell death in Drosophila cells, but not apoptosis induced by cell stress. We demonstrate that CARMER associates with the ecdysone receptor complex and modulates the ecdysone-induced transcription of a number of apoptotic genes. Thus, the chromatin-modifying protein, CARMER, modulates cell death by controlling the hormone-dependent expression of the core cell death machinery.

Original languageEnglish
Pages (from-to)18467-18471
Number of pages5
JournalJournal of Biological Chemistry
Volume279
Issue number18
DOIs
Publication statusPublished - 30 Apr 2004

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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