An anion-selective channel from the Pseudomonas aeruginosa outer membrane

Roland Benz, Michaela Gimple, Keith Poole, Robert E.W. Hancock

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17 Citations (Scopus)

Abstract

Protein P from Pseudomonas aeruginosa outer membrane was reconstituted in lipid bilayer membranes from diphytanoylphosphatidylcholine. The reconstitution resulted in the formation of anion-selective channels with a conductance of 160 pS for 0.1 M chloride solution. The channels were at least 100-times more selective for anions than for cations as judged from zero-current membrane potentials. The single-channel conductance was dependent on the size of the different anions and saturated at higher salt concentrations suggesting single ion occupancy of the protein P channel.

Original languageEnglish
Pages (from-to)387-390
Number of pages4
JournalBBA - Biomembranes
Volume730
Issue number2
DOIs
Publication statusPublished or Issued - 5 May 1983
Externally publishedYes

Keywords

  • (Ps. aeruginosa)
  • Anion selectivity
  • Channel formation
  • Lipid bilayer
  • Membrane reconstitution
  • Outer membrane

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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