Altered trafficking and turnover of LAMP-1 in pompe disease-affected cells

Peter J. Meikle, Miao Yan, Elaine M. Ravenscroft, Elizabeth L. Isaac, John J. Hopwood, Doug A. Brooks

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28 Citations (Scopus)

Abstract

The lysosome-associated membrane protein (LAMP-1) is elevated in the cells and plasma from lysosomal storage disorder-affected individuals; however, the mechanism of this elevation is not well defined. In this study we have investigated the synthesis, glycoprocessing, trafficking, and turnover of LAMP-1 in human skin fibroblasts from Pompe disease patients and control individuals. There were similar levels of LAMP-1 synthesis in both cell types, but glycoprocessing was retarded in Pompe (T(1/2) = 25 min) compared to control (T(1/2) = 17 min) fibroblasts. There was also a marked delay in trafficking of LAMP-1 to lysosomes of Pompe (T(1/2) = 200 rain) compared to control (T(1/2) = 100 min) cells. A proportion of newly synthesized LAMP-1 (5.4% in Pompe and 8.5% in controls) was trafficked out of the cell (T(1/2) = 3.5 h in controls) and, although significantly smaller than the lysosomal form, still had a transmembrane domain and cytoplasmic tail. In contrast, a soluble lysosomal pool of LAMP-1 had no tail sequence, suggesting that it had been clipped from the membrane. In turnover studies, LAMP-1 was more stable in Pompe (T(1/2) = 4.9 days) compared to control (T(1/2) = 1.6 days) cells, implying either reduced proteolysis or lysosomal function, in Pompe cells. These results indicate altered traffic and turnover of LAMP-1 in storage disorders and identify different intracellular and extracellular pools of soluble LAMP-1, suggesting alternative trafficking pathways.

Original languageEnglish
Pages (from-to)179-188
Number of pages10
JournalMolecular Genetics and Metabolism
Volume66
Issue number3
DOIs
Publication statusPublished or Issued - Mar 1999
Externally publishedYes

Keywords

  • LAMP-1
  • Lysosome
  • Pompe disease
  • Storage disorder

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

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