Activation of splanchnic and pelvic colonic afferents by bradykinin in mice

Stuart M. Brierley, R. C W Jones, L. Xu, G. F. Gebhart, L. A. Blackshaw

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66 Citations (Scopus)


Background: Lumbar splanchnic (LSN) and sacral pelvic (PN) nerves convey different mechanosensory information from the colon to the spinal cord. Here, we determined whether these pathways differ also in their chemosensitivity to bradykinin. Methods: Using a novel in vitro mouse colon preparation, serosal afferents were recorded from the LSN and PN and distinguished based on their mechanosensitivity to von Prey filaments (70-4000 mg) and insensitivity to colonic stretch (1-5 g) or fine mucosal stroking (10 mg). Bradykinin was applied into a ring around mechanoreceptive fields. Results: The LSN and PN afferents had different dynamic responses to mechanical stimuli: PN afferents required lower intensity stimuli, evoked larger responses, and displayed more maintained responses than LSN afferents. Bradykinin (1 μmol L-1) excited 66% (27 of 41) of LSN afferents. Responses to probing were potentiated after bradykinin. The concentration-dependent (EC50: 0.16 μmol L -1) response was reversed by the B2-receptor antagonist HOE-140 (10 nmol L-1). Twelve bradykinin responsive afferents were mechanically insensitive. More LSN serosal afferents responded to bradykinin than PN afferents (11%, P < 0.001), with larger responses (P < 0.05). No mechanically insensitive PN afferents were recruited by bradykinin. Conclusions: Bradykinin potently stimulates most splanchnic serosal afferents via B 2-receptors, but few pelvic afferents. Mechanically insensitive afferents recruited by bradykinin are exclusive to the LSN.

Original languageEnglish
Pages (from-to)854-862
Number of pages9
JournalNeurogastroenterology and Motility
Issue number6
Publication statusPublished or Issued - Dec 2005
Externally publishedYes


  • Afferents
  • B2 receptors
  • Bradykinin
  • Colon
  • Pelvic
  • Splanchnic

ASJC Scopus subject areas

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

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