A synthetic peptide substrate for initiation factor-2 kinases

Harry Mellor, Christopher G. Proud

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

A peptide P(45-56) corresponding to residues 45-56 (sequence: ILLSELSRRRIR) of eIF-2α was synthesised. It was phosphorylated by both of the well characterised eIF-2α kinases viz.; the heme-controlled repressor (HCR) and the double stranded RNA-dependent inhibitor (dsI). Of four other protein kinases tested only protein kinase C (PKC) phosphorylated P(45-56), with complete dependence on phosphatidylserine. Only the residue corresponding to serine-51 in eIF-2α was phosphorylated by HCR, dsI or PKC. The phosphorylation of the peptide by dsI and the phosphorylation of eIF-2α by dsI or HCR showed sigmoidal kinetics with respect to substrate concentration.

Original languageEnglish
Pages (from-to)430-437
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume178
Issue number2
DOIs
Publication statusPublished or Issued - 31 Jul 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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