A simple corneal perfusion chamber for drug penetration and toxicity studies

M. A. Thiel, N. Morlet, D. Schulz, H. F. Edelhauser, J. K. Dart, D. J. Coster, Keryn Williams

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19 Citations (Scopus)

Abstract

Aims - Corneal perfusion chambers are important tools in the development and assessment of ophthalmic drugs. The aim of this study was to design and test a modified perfusion chamber suitable for topical application of drugs to isolated corneoscleral preparations, and which allowed continuous monitoring of endothelial cell function. Methods - A polycarbonate and stainless steel perfusion chamber was designed to clamp corneas in a horizontal plane suitable for topical drug delivery. Endothelial cell function was assessed by ultrasonic pachymetry and specular microscopy during perfusion. Epithelial barrier function was assessed by penetration of fluorescein. Leakage was examined by measuring penetration of a large protein, IgG. Tissue architecture after perfusion was examined by conventional histology. Results - Corneas maintained a functionally and morphologically intact endothelial monolayer during perfusion periods of up to 14 hours. The epithelial harrier function was well preserved. The tissue clamp sealed the preparation effectively against leakage of macromolecules. Conclusion - The new chamber device forms a reliable tool for in vitro drug penetration and toxicity studies in isolated perfused corneoscleral tissue.

LanguageEnglish
Pages450-453
Number of pages4
JournalBritish Journal of Ophthalmology
Volume85
Issue number4
DOIs
Publication statusPublished - 18 Apr 2001
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Thiel, M. A., Morlet, N., Schulz, D., Edelhauser, H. F., Dart, J. K., Coster, D. J., & Williams, K. (2001). A simple corneal perfusion chamber for drug penetration and toxicity studies. British Journal of Ophthalmology, 85(4), 450-453. https://doi.org/10.1136/bjo.85.4.450