A randomized trial of supplementation with docosahexaenoic acid-rich tuna oil and its effects on the human milk cytokines interleukin 1β, interleukin 6, and tumor necrosis factor α

Joanna S. Hawkes, Dani Louise Bryan, Maria Makrides, Mark A. Neumann, Robert A. Gibson

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Increased consumption ot n-3 long-chain polyunsaturated fatty acids (PUFAs) has been recommended during pregnancy and lactation. The production of proinflammatory cytokines by human peripheral blood mononuclear cells (PBMCs) can be modified by dietary n-3 PUFAs. Objective: We sought to determine whether dietary supplementation of lactating women with docosahexaenoic acid (DHA) can modulate the concentration of cytokines in the aqueous phase of human milk and the production of cytokines by human milk cells (HMCs) and PBMCs. Design: In this double-blind, prospective, randomized trial, mothers of healthy full-term infants were asked to consume daily a nutritional supplement of 2000 mg oil containing either placebo (n = 40), 300 mg DHA + 70 mg eicosapentaenoic acid (EPA; n = 40), or 600 mg DHA + 140 mg EPA (n = 40). The fatty acid composition of plasma, PBMCs, milk, and HMCs from lactating mothers at 4 wk postpartum was measured by gas chromatography. The concentration of interleukin 6 and tumor necrosis factor a in milk and the production of interleukin 1β, tumor necrosis factor α, and interleukin 6 by PBMCs and HMCs after stimulation with lipopolysaccharide was measured by enzyme-linked immunosorbent assay. Results: At 4 wk postpartum, 82 mothers were still breast-feeding their infants. DHA increases in maternal plasma, PBMCs, milk, and HMCs were proportional to dietary DHA. There was no relation between tissue DHA status and cytokine concentrations. Conclusions: Consumption of ≤600 mg DHA and 140 mg EPA/d for 4 wk increased n-3 PUFA concentrations in relevant tissues but did not cause perturbations in cytokine concentrations in human milk.

LanguageEnglish
Pages754-760
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume75
Issue number4
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • Breast-feeding
  • Cytokines
  • Docosahexaenoic acid
  • Human milk
  • Human milk cells
  • Interleukin 1β
  • Interleukin 6
  • N-3 fatty acids
  • Randomized controlled trial
  • Tumor necrosis factor α
  • Women

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

@article{a1c0d5d3d4474d1889bf5ab44a5dab28,
title = "A randomized trial of supplementation with docosahexaenoic acid-rich tuna oil and its effects on the human milk cytokines interleukin 1β, interleukin 6, and tumor necrosis factor α",
abstract = "Background: Increased consumption ot n-3 long-chain polyunsaturated fatty acids (PUFAs) has been recommended during pregnancy and lactation. The production of proinflammatory cytokines by human peripheral blood mononuclear cells (PBMCs) can be modified by dietary n-3 PUFAs. Objective: We sought to determine whether dietary supplementation of lactating women with docosahexaenoic acid (DHA) can modulate the concentration of cytokines in the aqueous phase of human milk and the production of cytokines by human milk cells (HMCs) and PBMCs. Design: In this double-blind, prospective, randomized trial, mothers of healthy full-term infants were asked to consume daily a nutritional supplement of 2000 mg oil containing either placebo (n = 40), 300 mg DHA + 70 mg eicosapentaenoic acid (EPA; n = 40), or 600 mg DHA + 140 mg EPA (n = 40). The fatty acid composition of plasma, PBMCs, milk, and HMCs from lactating mothers at 4 wk postpartum was measured by gas chromatography. The concentration of interleukin 6 and tumor necrosis factor a in milk and the production of interleukin 1β, tumor necrosis factor α, and interleukin 6 by PBMCs and HMCs after stimulation with lipopolysaccharide was measured by enzyme-linked immunosorbent assay. Results: At 4 wk postpartum, 82 mothers were still breast-feeding their infants. DHA increases in maternal plasma, PBMCs, milk, and HMCs were proportional to dietary DHA. There was no relation between tissue DHA status and cytokine concentrations. Conclusions: Consumption of ≤600 mg DHA and 140 mg EPA/d for 4 wk increased n-3 PUFA concentrations in relevant tissues but did not cause perturbations in cytokine concentrations in human milk.",
keywords = "Breast-feeding, Cytokines, Docosahexaenoic acid, Human milk, Human milk cells, Interleukin 1β, Interleukin 6, N-3 fatty acids, Randomized controlled trial, Tumor necrosis factor α, Women",
author = "Hawkes, {Joanna S.} and Bryan, {Dani Louise} and Maria Makrides and Neumann, {Mark A.} and Gibson, {Robert A.}",
year = "2002",
language = "English",
volume = "75",
pages = "754--760",
journal = "American Journal of Clinical Nutrition",
issn = "0002-9165",
publisher = "American Society for Nutrition",
number = "4",

}

TY - JOUR

T1 - A randomized trial of supplementation with docosahexaenoic acid-rich tuna oil and its effects on the human milk cytokines interleukin 1β, interleukin 6, and tumor necrosis factor α

AU - Hawkes, Joanna S.

AU - Bryan, Dani Louise

AU - Makrides, Maria

AU - Neumann, Mark A.

AU - Gibson, Robert A.

PY - 2002

Y1 - 2002

N2 - Background: Increased consumption ot n-3 long-chain polyunsaturated fatty acids (PUFAs) has been recommended during pregnancy and lactation. The production of proinflammatory cytokines by human peripheral blood mononuclear cells (PBMCs) can be modified by dietary n-3 PUFAs. Objective: We sought to determine whether dietary supplementation of lactating women with docosahexaenoic acid (DHA) can modulate the concentration of cytokines in the aqueous phase of human milk and the production of cytokines by human milk cells (HMCs) and PBMCs. Design: In this double-blind, prospective, randomized trial, mothers of healthy full-term infants were asked to consume daily a nutritional supplement of 2000 mg oil containing either placebo (n = 40), 300 mg DHA + 70 mg eicosapentaenoic acid (EPA; n = 40), or 600 mg DHA + 140 mg EPA (n = 40). The fatty acid composition of plasma, PBMCs, milk, and HMCs from lactating mothers at 4 wk postpartum was measured by gas chromatography. The concentration of interleukin 6 and tumor necrosis factor a in milk and the production of interleukin 1β, tumor necrosis factor α, and interleukin 6 by PBMCs and HMCs after stimulation with lipopolysaccharide was measured by enzyme-linked immunosorbent assay. Results: At 4 wk postpartum, 82 mothers were still breast-feeding their infants. DHA increases in maternal plasma, PBMCs, milk, and HMCs were proportional to dietary DHA. There was no relation between tissue DHA status and cytokine concentrations. Conclusions: Consumption of ≤600 mg DHA and 140 mg EPA/d for 4 wk increased n-3 PUFA concentrations in relevant tissues but did not cause perturbations in cytokine concentrations in human milk.

AB - Background: Increased consumption ot n-3 long-chain polyunsaturated fatty acids (PUFAs) has been recommended during pregnancy and lactation. The production of proinflammatory cytokines by human peripheral blood mononuclear cells (PBMCs) can be modified by dietary n-3 PUFAs. Objective: We sought to determine whether dietary supplementation of lactating women with docosahexaenoic acid (DHA) can modulate the concentration of cytokines in the aqueous phase of human milk and the production of cytokines by human milk cells (HMCs) and PBMCs. Design: In this double-blind, prospective, randomized trial, mothers of healthy full-term infants were asked to consume daily a nutritional supplement of 2000 mg oil containing either placebo (n = 40), 300 mg DHA + 70 mg eicosapentaenoic acid (EPA; n = 40), or 600 mg DHA + 140 mg EPA (n = 40). The fatty acid composition of plasma, PBMCs, milk, and HMCs from lactating mothers at 4 wk postpartum was measured by gas chromatography. The concentration of interleukin 6 and tumor necrosis factor a in milk and the production of interleukin 1β, tumor necrosis factor α, and interleukin 6 by PBMCs and HMCs after stimulation with lipopolysaccharide was measured by enzyme-linked immunosorbent assay. Results: At 4 wk postpartum, 82 mothers were still breast-feeding their infants. DHA increases in maternal plasma, PBMCs, milk, and HMCs were proportional to dietary DHA. There was no relation between tissue DHA status and cytokine concentrations. Conclusions: Consumption of ≤600 mg DHA and 140 mg EPA/d for 4 wk increased n-3 PUFA concentrations in relevant tissues but did not cause perturbations in cytokine concentrations in human milk.

KW - Breast-feeding

KW - Cytokines

KW - Docosahexaenoic acid

KW - Human milk

KW - Human milk cells

KW - Interleukin 1β

KW - Interleukin 6

KW - N-3 fatty acids

KW - Randomized controlled trial

KW - Tumor necrosis factor α

KW - Women

UR - http://www.scopus.com/inward/record.url?scp=0036198505&partnerID=8YFLogxK

M3 - Article

VL - 75

SP - 754

EP - 760

JO - American Journal of Clinical Nutrition

T2 - American Journal of Clinical Nutrition

JF - American Journal of Clinical Nutrition

SN - 0002-9165

IS - 4

ER -