A panel of optimized primers and positive-control DNAs for PCR detection of common biological contaminants in mouse cell lines and tissue samples

Anne Marie Ayral, Sarah Clarkson, Michael Cheeseman, Sara Wells, T. Neil Dear

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

PCR-based testing for infectious agents in mouse cell lines and tissues has recently been developed as an alternative to the traditional MAP test. One drawback to currently available PCR-based assays is the lack of appropriate positive controls for PCR detection of the infectious agents. When negative samples are the norm and positive controls are absent, it is very difficult to feel confident detecting infectious agents. To alleviate this problem, the authors developed a panel of primers and positive-control DNA plasmids that enable rapid testing of biological samples, such as cell lines, tissues, or animal sera, for presence of the infectious agents most damaging to mouse colonies.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalLab Animal
Volume35
Issue number8
DOIs
Publication statusPublished - 1 Sep 2006

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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