A method to isolate and purify human bone marrow stromal stem cells

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Abstract

The STRO-1 antibody can be used as a single reagent to isolate human bone marrow stromal stem cells (BMSSC), owing to its restricted specificity to a cell surface molecule expressed by clonogenic BMSSC, with little or no reactivity to hematopoietic stem/progenitor populations or mature stromal elements. The present protocol uses a combination of two different immunoselection methodologies in an attempt to generate highly purified preparations of BMSSC. This process involves the initial isolation of a minor subpopulation of bone marrow mononuclear cells (approx 10%) expressing the STRO-1 antigen, by means of magnetic activated cell sorting. Dual-color fluorescence activated cell sorting is then used as a secondary step to further purify the rare STRO-1 bright expressing fraction that contains all of the colony-forming BMSSC, based on their co-expression of a secondary cell surface marker, CD106 (VCAM-1).

Original languageEnglish
Title of host publicationMesenchymal Stem Cells
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages45-57
Number of pages13
ISBN (Print)9781588297716
DOIs
Publication statusPublished - 1 Jan 2008

Publication series

NameMethods in Molecular Biology
Volume449
ISSN (Print)1064-3745

Keywords

  • Bone marrow stromal stem cells
  • Fluorescence activated cell sorting
  • Magnetic activated cell sorting
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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