A membrane protein primarily associated with the lysosomal compartment

Doug A. Brooks, Tessa M. Bradford, Sven R. Carlsson, John J. Hopwood

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A monoclonal antibody designated MBR 39 has been generated against a membrane associated protein found selectively on lysosomes. MBR 39 reacts with the cytosolic face of the lysosome and was used to develop an organelle binding assay which reacted with high density organelles characteristic of lysosomes. These organelles contained lysosomal enzyme markers which included the integral membrane protein acetyl-CoA: α-glucosaminide N-acetyltransferase and the soluble lysosomal enzyme markers acid phosphatase (mature form), β-hexosaminidase, arylsulfatase, and β-L-iduronidase. Under conditions which disrupt lysosomes the release of the latter soluble lysosomal enzymes was demonstrated from MBR 39 bound organelles. Immunoblots of MBR 39 with purified fibroblast lysosomal membrane, demonstrated reactivity with polypeptides of molecular mass 63 kDa (major species) and 73 kDa (minor species).

Original languageEnglish
Pages (from-to)162-170
Number of pages9
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1327
Issue number2
DOIs
Publication statusPublished or Issued - 25 Jul 1997

Keywords

  • Antibody
  • Biogenesis
  • Lysosome
  • Membrane protein
  • Monoclonal antibody
  • Organelle purification

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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