A DNA methylation fingerprint of 1628 human samples

Agustin F. Fernandez, Yassen Assenov, Jose Ignacio Martin-Subero, Balazs Balint, Reiner Siebert, Hiroaki Taniguchi, Hiroyuki Yamamoto, Manuel Hidalgo, Aik Choon Tan, Oliver Galm, Isidre Ferrer, Montse Sanchez-Cespedes, Alberto Villanueva, Javier Carmona, Jose V. Sanchez-Mut, Maria Berdasco, Victor Moreno, Gabriel Capella, David Monk, Esteban Ballestar & 18 others Santiago Ropero, Ramon Martinez, Marta Sanchez-Carbayo, Felipe Prosper, Xabier Agirre, Mario F. Fraga, Osvaldo Graña, Luis Perez-Jurado, Jaume Mora, Susana Puig, Jaime Prat, Lina Badimon, Annibale A. Puca, Stephen J. Meltzer, Thomas Lengauer, John Bridgewater, Christoph Bock, Manel Esteller

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

Most of the studies characterizing DNA methylation patterns have been restricted to particular genomic loci in a limited number of human samples and pathological conditions. Herein, we present a compromise between an extremely comprehensive study of a human sample population with an intermediate level of resolution of CpGs at the genomic level. We obtained a DNA methylation fingerprint of 1628 human samples in which we interrogated 1505 CpG sites. The DNA methylation patterns revealed show this epigenetic mark to be critical in tissue-type definition and stemness, particularly around transcription start sites that are not within a CpG island. For disease, the generated DNA methylation fingerprints show that, during tumorigenesis, human cancer cells underwent a progressive gain of promoter CpG-island hypermethylation and a loss of CpG methylation in non-CpG-island promoters. Although transformed cells are those in which DNA methylation disruption is more obvious, we observed that other common human diseases, such as neurological and autoimmune disorders, had their own distinct DNA methylation profiles. Most importantly, we provide proof of principle that the DNA methylation fingerprints obtained might be useful for translational purposes by showing that we are able to identify the tumor type origin of cancers of unknown primary origin (CUPs). Thus, the DNA methylation patterns identified across the largest spectrum of samples, tissues, and diseases reported to date constitute a baseline for developing higher-resolution DNA methylation maps and provide important clues concerning the contribution of CpG methylation to tissue identity and its changes in the most prevalent human diseases.

LanguageEnglish
Pages407-419
Number of pages13
JournalGenome Research
Volume22
Issue number2
DOIs
Publication statusPublished - 1 Feb 2012

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Fernandez, A. F., Assenov, Y., Martin-Subero, J. I., Balint, B., Siebert, R., Taniguchi, H., ... Esteller, M. (2012). A DNA methylation fingerprint of 1628 human samples. Genome Research, 22(2), 407-419. https://doi.org/10.1101/gr.119867.110
Fernandez, Agustin F. ; Assenov, Yassen ; Martin-Subero, Jose Ignacio ; Balint, Balazs ; Siebert, Reiner ; Taniguchi, Hiroaki ; Yamamoto, Hiroyuki ; Hidalgo, Manuel ; Tan, Aik Choon ; Galm, Oliver ; Ferrer, Isidre ; Sanchez-Cespedes, Montse ; Villanueva, Alberto ; Carmona, Javier ; Sanchez-Mut, Jose V. ; Berdasco, Maria ; Moreno, Victor ; Capella, Gabriel ; Monk, David ; Ballestar, Esteban ; Ropero, Santiago ; Martinez, Ramon ; Sanchez-Carbayo, Marta ; Prosper, Felipe ; Agirre, Xabier ; Fraga, Mario F. ; Graña, Osvaldo ; Perez-Jurado, Luis ; Mora, Jaume ; Puig, Susana ; Prat, Jaime ; Badimon, Lina ; Puca, Annibale A. ; Meltzer, Stephen J. ; Lengauer, Thomas ; Bridgewater, John ; Bock, Christoph ; Esteller, Manel. / A DNA methylation fingerprint of 1628 human samples. In: Genome Research. 2012 ; Vol. 22, No. 2. pp. 407-419.
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Fernandez, AF, Assenov, Y, Martin-Subero, JI, Balint, B, Siebert, R, Taniguchi, H, Yamamoto, H, Hidalgo, M, Tan, AC, Galm, O, Ferrer, I, Sanchez-Cespedes, M, Villanueva, A, Carmona, J, Sanchez-Mut, JV, Berdasco, M, Moreno, V, Capella, G, Monk, D, Ballestar, E, Ropero, S, Martinez, R, Sanchez-Carbayo, M, Prosper, F, Agirre, X, Fraga, MF, Graña, O, Perez-Jurado, L, Mora, J, Puig, S, Prat, J, Badimon, L, Puca, AA, Meltzer, SJ, Lengauer, T, Bridgewater, J, Bock, C & Esteller, M 2012, 'A DNA methylation fingerprint of 1628 human samples', Genome Research, vol. 22, no. 2, pp. 407-419. https://doi.org/10.1101/gr.119867.110

A DNA methylation fingerprint of 1628 human samples. / Fernandez, Agustin F.; Assenov, Yassen; Martin-Subero, Jose Ignacio; Balint, Balazs; Siebert, Reiner; Taniguchi, Hiroaki; Yamamoto, Hiroyuki; Hidalgo, Manuel; Tan, Aik Choon; Galm, Oliver; Ferrer, Isidre; Sanchez-Cespedes, Montse; Villanueva, Alberto; Carmona, Javier; Sanchez-Mut, Jose V.; Berdasco, Maria; Moreno, Victor; Capella, Gabriel; Monk, David; Ballestar, Esteban; Ropero, Santiago; Martinez, Ramon; Sanchez-Carbayo, Marta; Prosper, Felipe; Agirre, Xabier; Fraga, Mario F.; Graña, Osvaldo; Perez-Jurado, Luis; Mora, Jaume; Puig, Susana; Prat, Jaime; Badimon, Lina; Puca, Annibale A.; Meltzer, Stephen J.; Lengauer, Thomas; Bridgewater, John; Bock, Christoph; Esteller, Manel.

In: Genome Research, Vol. 22, No. 2, 01.02.2012, p. 407-419.

Research output: Contribution to journalArticle

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AU - Sanchez-Cespedes, Montse

AU - Villanueva, Alberto

AU - Carmona, Javier

AU - Sanchez-Mut, Jose V.

AU - Berdasco, Maria

AU - Moreno, Victor

AU - Capella, Gabriel

AU - Monk, David

AU - Ballestar, Esteban

AU - Ropero, Santiago

AU - Martinez, Ramon

AU - Sanchez-Carbayo, Marta

AU - Prosper, Felipe

AU - Agirre, Xabier

AU - Fraga, Mario F.

AU - Graña, Osvaldo

AU - Perez-Jurado, Luis

AU - Mora, Jaume

AU - Puig, Susana

AU - Prat, Jaime

AU - Badimon, Lina

AU - Puca, Annibale A.

AU - Meltzer, Stephen J.

AU - Lengauer, Thomas

AU - Bridgewater, John

AU - Bock, Christoph

AU - Esteller, Manel

PY - 2012/2/1

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Fernandez AF, Assenov Y, Martin-Subero JI, Balint B, Siebert R, Taniguchi H et al. A DNA methylation fingerprint of 1628 human samples. Genome Research. 2012 Feb 1;22(2):407-419. https://doi.org/10.1101/gr.119867.110