A chronic high fat diet alters the homologous and heterologous control of appetite regulating peptide receptor expression

Stephen J. Kentish, Gary A. Wittert, L. Ashley Blackshaw, Amanda J. Page

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16 Citations (Scopus)


Leptin, ghrelin and neuropeptide W (NPW) modulate vagal afferent activity, which may underlie theirappetite regulatory actions. High fat diet (HFD)-induced obesity induces changes in the plasma levels ofthese peptides and alters the expression of receptors on vagal afferents. We investigated homologousand heterologous receptor regulation by leptin, ghrelin and NPW. Mice were fed (12 weeks) a standardlaboratory diet (SLD) or HFD. Nodose ganglia were cultured overnight in the presence or absence ofeach peptide. Leptin (LepR), ghrelin (GHS-R), NPW (GPR7) and cholecystokinin type-1 (CCK1R) receptormRNA, and the plasma leptin, ghrelin and NPW levels were measured. SLD: leptin reduced LepR, GPR7,increased GHS-R and CCK1R mRNA; ghrelin increased LepR, GPR7, CCK1R, and decreased GHS-R. HFD:leptin decreased GHS-R and GPR7, ghrelin increased GHS-R and GPR7. NPW decreased all receptors exceptGPR7 which increased with HFD. Plasma leptin was higher and NPW lower in HFD. Thus, HFD-inducedobesity disrupts inter-regulation of appetite regulatory receptors in vagal afferents.

Original languageEnglish
Pages (from-to)150-158
Number of pages9
Publication statusPublished or Issued - 2013


  • CCK
  • Ghrelin
  • HFD-induced obesity
  • Leptin
  • NPW
  • Receptors

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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